Tested Applications
| Positive WB detected in | HeLa cells, HEK-293 cells, RAW 264.7 cells, PC-12 cells |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
86197-2-RR targets POLD1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag8178 Product name: Recombinant human POLD1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 858-1107 aa of BC008800 Sequence: EGAVAHAQDVISDLLCNRIDISQLVITKELTRAASDYAGKQAHVELAERMRKRDPGSAPSLGDRVPYVIISAAKGVAAYMKSEDPLFVLEHSLPIDTQYYLEQQLAKPLLRIFEPILGEGRAEAVLLRGDHTRCKTVLTGKVGGLLAFAKRRNCCIGCRTVLSHQGAVCEFCQPRESELYQKEVSHLNALEERFSRLWTQCQRCQGSLHEDVICTSRDCPIFYMRKKVRKDLEDQEQLLRRFGPPGPEAW Predict reactive species |
| Full Name | polymerase (DNA directed), delta 1, catalytic subunit 125kDa |
| Calculated Molecular Weight | 1107 aa, 124 kDa |
| Observed Molecular Weight | 124 kDa |
| GenBank Accession Number | BC008800 |
| Gene Symbol | POLD1 |
| Gene ID (NCBI) | 5424 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P28340 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
POLD1, also known as DNA polymerase subunit delta p125, is 1107 amino acid protein, which belongs to the DNA polymerase type-B family. POLD1 colocalizes with PCNA and POLD3 at S phase replication sites (PubMed:11595739). As the catalytic component of the trimeric (Pol-delta3 complex) and tetrameric DNA polymerase delta complexes (Pol-delta4 complex), plays a crucial role in high fidelity genome replication, including in lagging strand synthesis, and repair. A previous study demonstrated that overexpression of POLD1 is associated with an increased risk of breast cancer. (
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for POLD1 antibody 86197-2-RR | Download protocol |
| IHC protocol for POLD1 antibody 86197-2-RR | Download protocol |
| WB protocol for POLD1 antibody 86197-2-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
