Tested Applications
| Positive WB detected in | HeLa cells, HEK-293T cells, SH-SY5Y cells, HL-60, NIH/3T3 cells, mouse brain tissue, rat brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
86227-4-RR targets CUGBP1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag3677 Product name: Recombinant human CUGBP1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 107-479 aa of BC031079 Sequence: DRKLFIGMISKKCTENDIRVMFSSFGQIEECRILRGPDGLSRGCAFVTFTTRAMAQTAIKAMHQAQTMEGCSSPMVVKFADTQKDKEQKRMAQQLQQQMQQISAASVWGNLAGLNTLGPQYLALLQQTASSGNLNTLSSLHPMGGLNAMQLQNLAALAAAASAAQNTPSGTNALTTSSSPLSVLTSSAGSSPSSSSSNSVNPIASLGALQTLAGATAGLNVGSLAGMAALNGGLGSSGLSNGTGSTMEALTQAYSGIQQYAAAALPTLYNQNLLTQQSIGAAGSQKEGPEGANLFIYHLPQEFGDQDLLQMFMPFGNVVSAKVFIDKQTNLSKCFGFVSYDNPVSAQAAIQSMNGFQIGMKRLKVQLKRSKND Predict reactive species |
| Full Name | CUG triplet repeat, RNA binding protein 1 |
| Calculated Molecular Weight | 483 aa, 52 kDa |
| Observed Molecular Weight | 51-55 kDa |
| GenBank Accession Number | BC031079 |
| Gene Symbol | CUGBP1 |
| Gene ID (NCBI) | 10658 |
| RRID | AB_3744729 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q92879 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CUG triplet repeat RNA binding protein 1 (CUGBP1) is a member of the CUGBP Elav-like family (CELF), which plays an important role in cardiac development and function (PMID: 17130239). CUGBP1 has also been shown to localize to cytoplasmic stress granules when cells are stressed.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CUGBP1 antibody 86227-4-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The CUGBP1 Recombinant monoclonal antibody (250200A8), catalog number 86227-4-RR, has 1 total citation. The citation appears in Frontiers in Genetics (2025) and is associated with breast cancer research, specifically investigating how CELF1 promotes aerobic glycolysis and aggressive phenotypes in ER-positive breast cancer through GLUT1 regulation. The antibody was used for Western blot (WB) in human samples.
| Species | Application | Title |
|---|---|---|
Front Genet CELF1 promotes aerobic glycolysis and an aggressive phenotype in ER-positive breast cancer via GLUT1 regulation.
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