Tested Applications
| Positive WB detected in | HeLa cells, Jurkat cells, MCF-7 cells, SKOV-3 cells, K-562 cells |
| Positive IF/ICC detected in | SKOV-3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
86283-1-RR targets PDLIM7 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag4614 Product name: Recombinant human PDLIM7,LMP1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 3-289 aa of BC001093 Sequence: SFKVVLEGPAPWGFRLQGGKDFNVPLSISRLTPGGKAAQAGVAVGDWVLSIDGENAGSLTHIEAQNKIRACGERLSLGLSRAQPVQSKPQKASAPAADPPRYTFAPSVSLNKTARPFGAPPPADSAPQQNGQPLRPLVPDASKQRLMENTEDWRPRPGTGQSRSFRILAHLTGTEFMQDPDEEHLKKSSQVPRTEAPAPASSTPQEPWPGPTAPSPTSRPPWAVDPAFAERYAPDKTSTVLTRHSQPATPTPLQSRTSIVQAAAGGVPGGGSNNGKTPVCHQCHKVI Predict reactive species |
| Full Name | PDZ and LIM domain 7 (enigma) |
| Calculated Molecular Weight | 55/60 kDa |
| Observed Molecular Weight | 50 kDa |
| GenBank Accession Number | BC001093 |
| Gene Symbol | PDLIM7 |
| Gene ID (NCBI) | 9260 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9NR12 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PDLIM7 (PDZ and LIM domain protein 7), also known as ENIGMA or LIM mineralization protein 1 (LMP-1), is a member of PDZ-LIM proteins which function as scaffold to interact with multiple proteins through N-terminal PDZ domain and C-terminal LIM domain. Its LIM domains bind to proteins involved in mitogenic signaling, and it plays a role in tumorigenesis by promoting cancer cell invasion and metastasis. In addition, PDLIM7 is an essential positive regulator of osteoblast differentiation and maturation and bone formation.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for PDLIM7 antibody 86283-1-RR | Download protocol |
| WB protocol for PDLIM7 antibody 86283-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
