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  • Western Blot (WB) analysis of mouse liver tissue using AMDHD1 Recombinant antibody (86415-1-RR)

    WB analysis of mouse liver using 86415-1-RR

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 86415-1-RR (AMDHD1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using AMDHD1 Recombinant antibody (86415-1-RR)

    WB analysis using 86415-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86415-1-RR (AMDHD1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 86415-1-RR

    Affinity and Kinetic Characterization of 86415-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86415-1-RR against Human AMDHD1 were performed. The affinity constant is 9.59 nM.

AMDHD1 Recombinant monoclonal antibody

AMDHD1 Uni-rAb® Recombinant Antibody for WB, ELISA
Cat No. 86415-1-RR
Clone No.250819A2

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

Amidohydrolase domain-containing protein 1, EC:3.5.2.7, HMFT1272

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive WB detected inmouse liver tissue, rat liver tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

86415-1-RR targets AMDHD1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag15420

Product name: Recombinant human AMDHD1 protein

Source: e coli.-derived, T-HIS

Tag: 6*His

Domain: 78-426 aa of BC029146

Sequence: CILPGLVDAHTHPVWAGERVHEFAMKLAGATYMEIHQAGGGIHFTVERTRQATEEELFRSLQQRLQCMMRAGTTLVECKSGYGLDLETELKMLRVIERARRELDIGISATYCGAHSVPKGKTATEAADDIINNHLPKLKELGRNGEIHVDNIDVFCEKGVFDLDSTRRILQRGKDIGLQINFHGDELHPMKAAELGAELGAQAISHLEEVSDEGIVAMATARCSAILLPTTAYMLRLKQPRARKMLDEGVIVALGSDFNPNAYCFSMPMVMHLACVNMRMSMPEALAAATINAAYALGKSHTHGSLEVGKQGDLIIINSSRWEHLIYQFGGHHELIEYVIAKGKLIYKT

Predict reactive species
Full Name amidohydrolase domain containing 1
Calculated Molecular Weight 426 aa, 47 kDa
Observed Molecular Weight45-50 kDa
GenBank Accession NumberBC029146
Gene Symbol AMDHD1
Gene ID (NCBI) 144193
Conjugate Unconjugated
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ96NU7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

AMDHD1 is a protein-coding gene that encodes the enzyme imidazolonepropionase. It is involved in the histidine catabolic process and has one potential isoform. The protein of AMDHD1 is located in the cytoplasm, and is expressed in all tissues, mainly in the liver, kidney and adrenal gland.

Protocols

Product Specific Protocols
WB protocol for AMDHD1 antibody 86415-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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