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IL22RA2 Recombinant monoclonal antibody, PBS Only (Detector) (86665-1-PBS)

IL22RA2 Recombinant monoclonal antibody, PBS Only (Detector)

Name: IL22RA2 Recombinant monoclonal antibody, PBS Only (Detector), 100ug
Brand: Proteintech
SKU: 86665-1-PBS-100UG

IL22RA2 Uni-rAb^® Recombinant Antibody for Sandwich ELISA, Indirect ELISA

Cat No. 86665-1-PBS

Clone No.250314G9

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Sandwich ELISA, Indirect ELISA

Synonyms

IL-22RA2, CRF2 10, CRF2 S1, CRF2-10, CRF2-S1

Formulation: PBS Only

PBS Only

Conjugate: Unconjugated

Size/Concentration:

SKU:

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Filter: AllSandwich ELISA sample testSandwich ELISAAffinity

Sandwich ELISA sample test of MP02591-1
Plasma of six individual healthy human donors was measured. The IL-22RA2 concentration of detected samples was determined to be 0.85 ng/mL with a range of 0.38-2.04 ng/mL.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
Sandwich ELISA sample test of MP02591-1
The mean IL-22RA2 concentration was determined to be 9.02 ng/mL in U-937 cell extract based on a 2.60 mg/mL extract load and 51.68 ng/mL in Raji cell extract based on a 1.30 mg/mL extract load.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
Sandwich ELISA standard curve of MP02591-1
Sandwich ELISA standard curve of MP02591-1, Human IL-22RA2 Recombinant Matched Antibody Pair - PBS only. 86665-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg2825. 86665-1-PBS was HRP conjugated as the detection antibody. Range: 0.781-50 ng/mL.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
Affinity of 86665-1-PBS
Biolayer interferometry (BLl) kinetic assay of 86665-1-PBS against Human IL22RA2 was performed. The affinity constant is 37.5 nM.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

86665-1-PBS targets IL22RA2 as part of a matched antibody pair:

MP02591-1: 86665-2-PBS capture and 86665-1-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Eg2825 Product name: Recombinant Human IL-22RA2 protein (rFc Tag) Source: mammalian cells-derived, V37 Tag: C-rFc Domain: 22-231 aa of NM_181309.1 Sequence: TQSTHESLKPQRVQFQSRNFHNILQWQPGRALTGNSSVYFVQYKIYGQRQWKNKEDCWGTQELSCDLTSETSDIQEPYYGRVRAASAGSYSEWSMTPRFTPWWETKIDPPVMNITQVNGSLLVILHAPNLPYRYQKEKNVSIEDYYELLYRVFIINNSLEKEQKVYEGAHRAVEIEALTPHSSYCVVAEIYQPMLDRRSQRSEERCVEIP Predict reactive species
Full Name	interleukin 22 receptor, alpha 2
Calculated Molecular Weight	27 kDa
GenBank Accession Number	NM_181309.1
Gene Symbol	IL-22RA2
Gene ID (NCBI)	116379
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q969J5-2
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.