Tested Applications
| Positive WB detected in | HeLa cells, HepG2 cells, HSC-T6 cells, K-562 cells, NIH/3T3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
86693-2-RR targets NQO1 in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag2009 Product name: Recombinant human NQO1 protein Source: e coli.-derived, PKG Tag: GST Domain: 1-274 aa of BC007659 Sequence: MVGRRALIVLAHSERTSFNYAMKEAAAAALKKKGWEVVESDLYAMNFNPIISRKDITGKLKDPANFQYPAESVLAYKEGHLSPDIVAEQKKLEAADLVIFQFPLQWFGVPAILKGWFERVFIGEFAYTYAAMYDKGPFRSKKAVLSITTGGSGSMYSLQGIHGDMNVILWPIQSGILHFCGFQVLEPQLTYSIGHTPADARIQILEGWKKRLENIWDETPLYFAPSSLFDLNFQAGFLMKKEVQDEEKNKKFGLSVGHHLGKSIPTDNQIKARK Predict reactive species |
| Full Name | NAD(P)H dehydrogenase, quinone 1 |
| Calculated Molecular Weight | 274 aa, 31 kDa |
| Observed Molecular Weight | 31 kDa |
| GenBank Accession Number | BC007659 |
| Gene Symbol | NQO1 |
| Gene ID (NCBI) | 1728 |
| RRID | AB_3745057 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P15559 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
NQO1, also named as DIA4, NMOR1, DTD and QR1, belongs to the NAD(P)H dehydrogenase (quinone) family. This enzyme apparently serves as a quinone reductase in connection with conjugation reactions of hydroquinons involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis. It is known to be involved in benzene metabolism. In human studies of ozone exposure, polymorphisms in oxidative stress genes (NQO1, GSTM1, GSTP1) modify respiratory symptoms, lung function, biomarkers and risk of asthma. (PMID:18511640, PMID:18848868)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for NQO1 antibody 86693-2-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The NQO1 Recombinant Monoclonal Antibody (251713C3), catalog number 86693-2-RR, has 21 citations. These appear in journals including Advanced Science, Phytomedicine, Free Radical Biology and Medicine, International Journal of Biological Macromolecules, Journal of Molecular Cell Biology, European Journal of Medicinal Chemistry, International Journal of Molecular Sciences, Antioxidants, Foods, Nutrients, Biochemical and Biophysical Research Communications, and others. Research fields span Nrf2 signaling, oxidative stress, mitochondrial dysfunction, neuroprotection, liver injury, ferroptosis, inflammation, NAFLD, pulmonary fibrosis, and hypertension.
| Species | Application | Title |
|---|---|---|
Adv Sci (Weinh) Procyanidin Capsules Combat ALF by Restoring Mitochondrial Homeostasis and Inhibiting Necroptosis via the PGAM5/DRP1/PINK1 Pathway. | ||
Phytomedicine Farrerol mitigates fungal keratitis by enhancing fungal clearance and limiting inflammation through autophagy and Nrf2 pathway. | ||
Phytomedicine Cannabidiol attenuates methamphetamine-induced psychosis via anti-oxidative stress: σ1R-mediated mitochondrial dysfunction as a critical pathway. | ||
Int J Biol Macromol Effects of different deproteinization methods on the hepatoprotective activity of Smilax china L. polysaccharides against CCl(4)-induced acute liver injury in mice. | ||
Int J Biol Macromol The gut-liver axis mediates the protective effect of Cinnamomum chago polysaccharide against acute liver injury: Insights from integrated microbiome and metabolomics analysis. | ||
Antioxidants (Basel) Propyl Gallate Attenuates Cognitive Deficits Induced by Chronic Sleep Deprivation Through Nrf2 Activation and NF-κB Inhibition. |
