Home
Products
Primary Antibodies
Recombinant Antibodies
Cyclophilin B Recombinant monoclonal antibody (86744-1-RR)

Cyclophilin B Recombinant monoclonal antibody

Cyclophilin B Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 86744-1-RR

Clone No.251476F7

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

PPIB, CYP S1, CYPB, CYP-S1, EC:5.2.1.8

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

SKU:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

Proteintech Guarantee

Filter: AllWB

WB analysis using 86744-1-RR
Various lysates were subjected to SDS PAGE followed by western blot with 86744-1-RR (Cyclophilin B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis using 86744-1-RR
Various lysates were subjected to SDS PAGE followed by western blot with 86744-1-RR (Cyclophilin B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Overview

Tested Applications
Product Information
Protocols

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in

NIH/3T3 cells, Jurkat cells, Raji cells, mouse liver tissue, rat liver tissue

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

86744-1-RR targets Cyclophilin B in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Ag2161 Product name: Recombinant human PPIB protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-216 aa of BC032138 Sequence: MLRLSERNMKVLLAAALIAGSVFFLLLPGPSAADEKKKGPKVTVKVYFDLRIGDEDVGRVIFGLFGKTVPKTVDNFVALATGEKGFGYKNSKFHRVIKDFMIQGGDFTRGDGTGGKSIYGERFPDENFKLKHYGPGWVSMANAGKDTNGSQFFITTVKTAWLDGKHVVFGKVLEGMEVVRKVESTKTDSRDKPLKDVIIADCGKIEVEKPFAIAKE Predict reactive species
Full Name	peptidylprolyl isomerase B (cyclophilin B)
Calculated Molecular Weight	216 aa, 24 kDa
Observed Molecular Weight	19-24 kDa
GenBank Accession Number	BC032138
Gene Symbol	Cyclophilin B
Gene ID (NCBI)	5479
RRID	AB_3745097
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P23284
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cyclophilin B (CYPB) , also named as PPIB, SCYLP, PPIase B and CYP-S1, belongs to the cyclophilin-type PPIase family and PPIase B subfamily. PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. PPIB is secreted in biological fluids such as blood or milk and binds to a specific receptor present on the human lymphoblastic cell line Jurkat and on human peripheral blood lymphocytes. The full-length of PPIB has 216aa (24 kDa), and a 33aa-signal peptide could be removed in the matrue form (about 19 kDa).