• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
Use Able AI chat for product recommendations
Filter:
  • Western Blot (WB) analysis of various lysates using ANKRD46 Recombinant monoclonal antibody (86827-4-RR)

    WB analysis using 86827-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86827-4-RR (ANKRD46 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using ANKRD46 Recombinant monoclonal antibody (86827-4-RR)

    WB analysis using 86827-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86827-4-RR (ANKRD46 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using ANKRD46 Recombinant monoclonal antibody (86827-4-RR)

    IF Staining of HEK-293 using 86827-4-RR

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using ANKRD46 antibody (86827-4-RR, Clone: 251840D6 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Immunofluorescence (IF) / fluorescent staining of Jurkat cells using ANKRD46 Recombinant monoclonal antibody (86827-4-RR)

    IF Staining of Jurkat using 86827-4-RR

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using ANKRD46 antibody (86827-4-RR, Clone: 251840D6 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 86827-4-RR

    Affinity and Kinetic Characterization of 86827-4-RR

    Biolayer interferometry (BLl) kinetic assays of 86827-4-RR against Human ANKRD46 were performed. The affinity constant is 0.351 nM.

ANKRD46 Recombinant monoclonal antibody

ANKRD46 Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 86827-4-RR
Clone No.251840D6

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

ANK-S, ankyrin repeat domain 46, Ankyrin repeat domain-containing protein 46

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

Please visit your regions distributor:

Tested Applications

Positive WB detected inHeLa cells, mouse brain tissue, MDA-MB-231 cells，HepG2 cells, Jurkat cells, rat brain tissue
Positive IF/ICC detected inJurkat cells, HEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

86827-4-RR targets ANKRD46 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag26623

Product name: Recombinant human ANKRD46 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-189 aa of BC060843

Sequence: MSYVFVNDSSQTNVPLLQACIDGDFNYSKRLLESGFDPNIRDSRGRTGLHLAAARGNVDICQLLHKFGADLLATDYQGNTALHLCGHVDTIQFLVSNGLKIDICNHQGATPLVLAKRRGVNKDVIRLLESLEEQEVKGFNRGTHSKLETMQTAESESAMESHSLLNPNLQQGEGVLSSFRTTWQEFVED

Predict reactive species
Full Name ankyrin repeat domain 46
Observed Molecular Weight 25 kDa
GenBank Accession NumberBC060843
Gene Symbol ANKRD46
Gene ID (NCBI) 157567
Conjugate Unconjugated
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ86W74
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ANKRD46 encodes a protein containing multiple ankyrin repeats. Ankyrin domains function in protein-protein interactions in a variety of cellular processes. ANKRD46 also known as ankyrin repeat small protein (ANK-S), is a 228-amino acid single-pass membrane protein(PMID: 21219636).

Protocols

Product Specific Protocols
IF protocol for ANKRD46 antibody 86827-4-RRDownload protocol
WB protocol for ANKRD46 antibody 86827-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
||
New chat

Able™

正在加载，请稍候...