Tested Applications
| Positive WB detected in | Daudi cells, Raji cells, Jurkat cells |
| Positive IF/ICC detected in | Jurkat cells |
| Positive ChIP-qPCR detected in | Raji cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| CHIP-QPCR | CHIP-QPCR : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
86937-1-RR targets FOXP1 in WB, IF/ICC, ELISA, ChIP-qPCR applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag17045 Product name: Recombinant human FOXP1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 349-457 aa of BC131720 Sequence: QLELQLAKDKERLQAMMTHLHVKSTEPKAAPQPLNLVSSVTLSKSASEASPQSLPHTPTTPTAPLTPVTQGPSVITTTSMHTVGPIRRRYSDKYNVPISSADIAQNQEF Predict reactive species |
| Full Name | forkhead box P1 |
| Calculated Molecular Weight | 677 aa, 75 kDa |
| Observed Molecular Weight | 75-77 kDa, 65-67 kDa |
| GenBank Accession Number | BC131720 |
| Gene Symbol | FOXP1 |
| Gene ID (NCBI) | 27086 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9H334 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
FOXP1, also known as Mac-1-regulated forkhead, is a 677 amino acid protein, which forms homodimers and heterodimers with FOXP2 and FOXP4 (PubMed:25027557). Dimerization is required for DNA-binding. FOXP1 has an important function in neuronal development.9 Mutations of its gene, FOXP1, located on chromosome 3p14.1,7 can result in the development of autism spectrum disorder, intellectual disability, speech and language deficits as well as motor development delay. FOXP1 is also engaged in lung and esophagus morphogenesis, as well as in B-cell development.7,10 The widely researched role of FOXP1 in carcinogenesis is of great importance, although still unclear to some extent. FOXP1 exists some isoforms with MV 75-77 kDa, 65-67 kDa, 12 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for FOXP1 antibody 86937-1-RR | Download protocol |
| WB protocol for FOXP1 antibody 86937-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
