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CNPase Recombinant monoclonal antibody (86967-4-RR)

CNPase Recombinant monoclonal antibody

CNPase Uni-rAb^® Recombinant Antibody for WB, IHC, IF-P, ELISA

Cat No. 86967-4-RR

Clone No.252105D1

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Synonyms

CNP, 2',3' cyclic nucleotide 3' phosphodiesterase, 2',3'-cyclic-nucleotide 3'-phosphodiesterase, EC:3.1.4.37

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

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Quantity

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WB analysis using 86967-4-RR
Various lysates were subjected to SDS PAGE followed by western blot with 86967-4-RR (CNP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis using 86967-4-RR
Various lysates were subjected to SDS PAGE followed by western blot with 86967-4-RR (CNP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse brain using 86967-4-RR
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 86967-4-RR (CNP antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of mouse brain using 86967-4-RR
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using CNPase antibody (86967-4-RR, Clone: 252105D1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of mouse brain using 86967-4-RR
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using CNPase antibody (86967-4-RR, Clone: 252105D1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Overview

Tested Applications
Product Information
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	HepG2 cells, mouse brain tissue, A375 cells, A431 cells, rat brain tissue
Positive IHC detected in	mouse brain tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	mouse brain tissue

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:10000
Immunohistochemistry (IHC)	IHC : 1:1000-1:4000
Immunofluorescence (IF)-P	IF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

86967-4-RR targets CNPase in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Ag4227 Product name: Recombinant human CNP protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-421 aa of BC028040 Sequence: MNRGFSRKSHTFLPKIFFRKMSSSGAKDKPELQFPFLQDEDTVATLLECKTLFILRGLPGSGKSTLARVIVDKYRDGTKMVSADAYKITPGARGAFSEEYKRLDEDLAAYCRRRDIRILVLDDTNHERERLEQLFEMADQYQYQVVLVEPKTAWRLDCAQLKEKNQWQLSADDLKKLKPGLEKDFLPLYFGWFLTKKSSETLRKAGQVFLEELGNHKAFKKELRQFVPGDEPREKMDLVTYFGKRPPGVLHCTTKFCDYGKAPGAEEYAQQDVLKKSYSKAFTLTISALFVTPKTTGARVELSEQQLQLWPSDVDKLSPTDNLPRGSRAHITLGCAADVEAVQTGLDLLEILRQEKGGSRGEEVGELSRGKLYSLGNGRWMLTLAKNMEVRAIFTGYYGKGKPVPTQGSRKGGALQSCTII Predict reactive species
Full Name	2',3'-cyclic nucleotide 3' phosphodiesterase
Calculated Molecular Weight	421 aa, 48 kDa
Observed Molecular Weight	45-48 kDa
GenBank Accession Number	BC028040
Gene Symbol	CNPase
Gene ID (NCBI)	1267
RRID	AB_3745239
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P09543
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IF protocol for CNPase antibody 86967-4-RR	Download protocol
IHC protocol for CNPase antibody 86967-4-RR	Download protocol
WB protocol for CNPase antibody 86967-4-RR	Download protocol