Tested Applications
| Positive WB detected in | human Neuregulin-1 treated T-47D cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
87096-1-RR targets Phospho-HER2/ErbB2 (Tyr1248) in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian) |
| Calculated Molecular Weight | 1255 aa, 138 kDa |
| Observed Molecular Weight | 185 kDa |
| GenBank Accession Number | BC156755 |
| Gene Symbol | HER2/ErbB2 |
| Gene ID (NCBI) | 2064 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P04626 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
HER2, also known as ErbB2 and Neu, is a 185-kDa transmembrane glycoprotein that is a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. Phospho-HER2/ErbB2 (Tyr1248) is a phosphorylated form of the HER2 protein, which is a member of the epidermal growth factor receptor (EGFR) family. This phosphorylation event is considered a prerequisite for HER2 signaling and its transforming activity. The phosphorylation of HER2 at Tyr1248 residue is a key step in the activation of downstream signaling cascades that regulate various cellular processes, including cell growth, proliferation, and survival. Tyrosine 1248 is the main autophosphorylation site in the carboxy terminal domain of ErbB2 linked to downstream signaling through the ras-raf-MEK-p42/44 MAPK signal transduction pathway. (PMID: 19084911)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Phospho-HER2/ErbB2 (Tyr1248) antibody 87096-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
