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Complement Factor I Recombinant monoclonal antibody (87109-1-RR)

Complement Factor I Recombinant monoclonal antibody

Complement Factor I Uni-rAb^® Recombinant Antibody for WB, IHC, ELISA

Cat No. 87109-1-RR

Clone No. 252199E11

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Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

C3B/C4B inactivator, CFI, Complement factor I heavy chain, Complement factor I light chain, EC:3.4.21.45

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Unconjugated

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Filter: AllWBIHC

WB analysis using 87109-1-RR
Various lysates were subjected to SDS PAGE followed by western blot with 87109-1-RR (CFI antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human liver using 87109-1-RR
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 87109-1-RR (Complement Factor I antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human liver using 87109-1-RR
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 87109-1-RR (Complement Factor I antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Overview

Tested Applications
Product Information
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MSDS SDS

Tested Applications

Positive WB detected in	HepG2 cells, HuH-7 cells, U-251 cells
Positive IHC detected in	human liver tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunohistochemistry (IHC)	IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

87109-1-RR targets Complement Factor I in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Eg3066 Product name: Recombinant Human Complement Factor I protein (rFc Tag) Source: mammalian cells-derived, V37 Tag: C-rFc Domain: 19-583 aa of NM_000204.5 Sequence: KVTYTSQEDLVEKKCLAKKYTHLSCDKVFCQPWQRCIEGTCVCKLPYQCPKNGTAVCATNRRSFPTYCQQKSLECLHPGTKFLNNGTCTAEGKFSVSLKHGNTDSEGIVEVKLVDQDKTMFICKSSWSMREANVACLDLGFQQGADTQRRFKLSDLSINSTECLHVHCRGLETSLAECTFTKRRTMGYQDFADVVCYTQKADSPMDDFFQCVNGKYISQMKACDGINDCGDQSDELCCKACQGKGFHCKSGVCIPSQYQCNGEVDCITGEDEVGCAGFASVTQEETEILTADMDAERRRIKSLLPKLSCGVKNRMHIRRKRIVGGKRAQLGDLPWQVAIKDASGITCGGIYIGGCWILTAAHCLRASKTHRYQIWTTVVDWIHPDLKRIVIEYVDRIIFHENYNAGTYQNDIALIEMKKDGNKKDCELPRSIPACVPWSPYLFQPNDTCIVSGWGREKDNERVFSLQWGEVKLISNCSKFYGNRFYEKEMECAGTYDGSIDACKGDSGGPLVCMDANNVTYVWGVVSWGENCGKPEFPGVYTKVANYFDWISYHVGRPFISQYNV Predict reactive species
Full Name	complement factor I
Calculated Molecular Weight	66 kDa
Observed Molecular Weight	75-80 kDa
GenBank Accession Number	NM_000204.5
Gene Symbol	CFI
Gene ID (NCBI)	3426
RRID	AB_3745346
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P05156
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Complement Factor I (CFI) protein is a key plasma serine protease, which is mainly synthesized in the liver, with a molecular weight of about 75-80 kDa, and circulates in the blood in the form of heterodimers of heavy chain (51 kDa)- light chain (37 kDa) disulfide bonds.