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  • Western Blot (WB) analysis of various lysates using PSMC3 Recombinant monoclonal antibody (87128-4-RR)

    WB analysis using 87128-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 87128-4-RR (PSMC3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using PSMC3 Recombinant monoclonal antibody (87128-4-RR)

    IF Staining of HepG2 using 87128-4-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PSMC3 antibody (87128-4-RR, Clone: 252375C1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity of 87128-4-RR

    Affinity of 87128-4-RR

    Biolayer interferometry (BLl) kinetic assays of 87128-4-RR against Human PSMC3 were performed. The affinity constant is below 1 pM.

PSMC3 Recombinant monoclonal antibody

PSMC3 Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 87128-4-RR
Clone No.252375C1

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

26S proteasome AAA-ATPase subunit RPT5, 26S proteasome regulatory subunit 6A, Tat-binding protein 1, TBP1, TBP-1

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive WB detected inHEK-293 cells, HepG2 cells, Jurkat cells, T-47D cells, U2OS cells
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

87128-4-RR targets PSMC3 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag21408

Product name: Recombinant human PSMC3 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-200 aa of BC008713

Sequence: MNLLPNIESPVTRQEKMATVWDEAEQDGIGEEVLKMSTEEIIQRTRLLDSEIKIMKSEVLRVTHELQAMKDKIKENSEKIKVNKTLPYLVSNVIELLDVDPNDQEEDGANIDLDSQRKGKCAVIKTSTRQTYFLPVIGLVDAEKLKPGDLVGVNKDSYLILETLPTEYDSRVKAMEVDERPTEQYSDIGGLDKQIQELVE

Predict reactive species
Full Name proteasome (prosome, macropain) 26S subunit, ATPase, 3
Calculated Molecular Weight 49 kDa
Observed Molecular Weight 49 kDa
GenBank Accession NumberBC008713
Gene Symbol PSMC3
Gene ID (NCBI) 5702
Conjugate Unconjugated
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP17980
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IF protocol for PSMC3 antibody 87128-4-RRDownload protocol
WB protocol for PSMC3 antibody 87128-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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