Tested Applications
| Positive WB detected in | Daudi cells, Ramos cells, Raji cells |
| Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | THP-1 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
87173-1-RR targets LY86 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Eg3108 Product name: recombinant human LY86 protein Source: mammalian cells-derived, V37 Tag: C-rFc Domain: 21-162 aa of NM_004271.4 Sequence: GGGGKAWPTHVVCSDSGLEVLYQSCDPLQDFGFSVEKCSKQLKSNINIRFGIILREDIKELFLDLALMSQGSSVLNFSYPICEAALPKFSFCGRRKGEQIYYAGPVNNPEFTIPQGEYQVLLELYTEKRSTVACANATIMCS Predict reactive species |
| Full Name | lymphocyte antigen 86 |
| Calculated Molecular Weight | 18 kDa |
| Observed Molecular Weight | 20 kDa |
| GenBank Accession Number | NM_004271.4 |
| Gene Symbol | LY86 |
| Gene ID (NCBI) | 9450 |
| RRID | AB_3745388 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | O95711 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
LY86, also known as protein MD-1, is a secreted glycoprotein that is associated with RP105 (TLR family proteins) and plays a significant role in the B cell surface expression of RP105. The RP105/MD-1 complex is expressed on immune cells, including B cells, macrophages, and dendritic cells (PMID: 37861500). By forming the RP105-MD-1 complex, LY86 fine-tunes recognition of bacterial lipopolysaccharide (LPS) and subsequent cytokine production, acting as both a facilitator and a brake on innate immune responses. LY86 influences sterile inflammatory conditions such as atherosclerosis, obesity, insulin resistance and cardiac remodeling, and is being explored as a biomarker for unstable carotid plaques and other immune-mediated diseases (PMID: 39085350).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for LY86 antibody 87173-1-RR | Download protocol |
| IHC protocol for LY86 antibody 87173-1-RR | Download protocol |
| WB protocol for LY86 antibody 87173-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
