Tested Applications
| Positive WB detected in | HeLa cells, H9C2 cells, mouse heart tissue, HEK-293 cells, MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
87311-1-RR targets INPP5F in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag39933 Product name: Recombinant human INPP5F protein Source: e coli.-derived, PET30a Tag: 6*His Domain: 788-1132 aa of BC111493 Sequence: VKQTKSNVNIGNLRKLGNFTKPEMKVNFLKPNLKVNLWKSDSSLETMENTGVMDKVQAESDGDMSSDNDSYHSDEFLTNSKSDEDRQLANSLESVGPIDYVLPSCGIIASAPRLGSRSQSLSSTDSSVHAPSEITVAHGSGLGKGQESPLKKSPSAGDVHILTGFAKPMDIYCHRFVQDAQNKVTHLSETRSVSQQASQERNQMTNQVSDETQSESTEQTPSRPSQLDVSLSATGPQFLSVEPAHSVASQKTPTSASSMLELETGLHVTPSPSESSSSRAVSPFAKIRSSMVQVASITQAGLTHGINFAVSKVQKSPPEPEIINQVQQNELKKMFIQCQTRIIQI Predict reactive species |
| Full Name | inositol polyphosphate-5-phosphatase F |
| Calculated Molecular Weight | 1132 aa, 128 kDa |
| Observed Molecular Weight | 128-140 kDa |
| GenBank Accession Number | BC111493 |
| Gene Symbol | INPP5F |
| Gene ID (NCBI) | 22876 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9Y2H2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
INPP5F can negatively regulates STAT3 signaling pathway through inhibition of STAT3 phosphorylation and translocation to the nucleus.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for INPP5F antibody 87311-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
