Home
Products
Primary Antibodies
Recombinant Antibodies
GFRA1 Recombinant monoclonal antibody (87383-1-RR)

GFRA1 Recombinant monoclonal antibody

GFRA1 Uni-rAb^® Recombinant Antibody for WB, IHC, ELISA

Cat No. 87383-1-RR

Clone No. 252616C9

Review Product

Download datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

GDNF family receptor alpha 1, GDNF family receptor alpha-1, GDNF receptor alpha 1, GDNF receptor alpha-1, GDNFR

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

SKU:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

Proteintech Guarantee

Filter: AllWBIHCAffinity

WB analysis using 87383-1-RR
Various lysates were subjected to SDS PAGE followed by western blot with 87383-1-RR (GFRA1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse brain using 87383-1-RR
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 87383-1-RR (GFRA1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
Affinity of 87383-1-RR
Biolayer interferometry (BLl) kinetic assays of 87383-1-RR against Human GFRA1 were performed. The affinity constant is below 1 pM.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Able AI

What's the best dilution for GFRA1 in ?

Overview

Tested Applications
Product Information
Protocols

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	MCF-7 cells, ACHN cells
Positive IHC detected in	mouse brain tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:6000
Immunohistochemistry (IHC)	IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

87383-1-RR targets GFRA1 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Eg5427 Product name: Recombinant Human GFRA1 protein (rFc Tag) Source: mammalian cells-derived, V37 Tag: C-rFc Domain: 25-429 aa of NM_005264.8 Sequence: DRLDCVKASDQCLKEQSCSTKYRTLRQCVAGKETNFSLASGLEAKDECRSAMEALKQKSLYNCRCKRGMKKEKNCLRIYWSMYQSLQGNDLLEDSPYEPVNSRLSDIFRVVPFISDVFQQVEHIPKGNNCLDAAKACNLDDICKKYRSAYITPCTTSVSNDVCNRRKCHKALRQFFDKVPAKHSYGMLFCSCRDIACTERRRQTIVPVCSYEEREKPNCLNLQDSCKTNYICRSRLADFFTNCQPESRSVSSCLKENYADCLLAYSGLIGTVMTPNYIDSSSLSVAPWCDCSNSGNDLEECLKFLNFFKDNTCLKNAIQAFGNGSDVTVWQPAFPVQTTTATTTTALRVKNKPLGPAGSENEIPTHVLPPCANLQAQKLKSNVSGNTHLCISNGNYEKEGLGASS Predict reactive species
Full Name	GDNF family receptor alpha 1
Calculated Molecular Weight	51 kDa
Observed Molecular Weight	55 kDa
GenBank Accession Number	NM_005264.8
Gene Symbol	GFRA1
Gene ID (NCBI)	2674
RRID	AB_3745502
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P56159-1
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GFRA1, also named as GDNFRA, RETL1 and TRNR1, is a receptor for GDNF. It mediates the GDNF-induced autophosphorylation and activation of the RET receptor. GFRA1 has two isoforms with MW 45-50 kDa and 50-55 kDa.