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Phospho-NPM1 (Thr199) Recombinant monoclonal antibody (87394-1-RR)

Phospho-NPM1 (Thr199) Recombinant monoclonal antibody

Phospho-NPM1 (Thr199) Uni-rAb^® Recombinant Antibody for WB, FC (Intra), ELISA

Cat No. 87394-1-RR

Clone No.252639G7

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Synonyms

NPM1 (Thr199), Phospho NPM1 (Thr199), Phospho-NPM, Phospho-NPM (Thr199), Phospho-NPM1

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

SKU:

-/ -

Freight/Packing: -

Quantity

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Filter: AllWBSEC-HPLCFC (Intra)

WB analysis of HeLa using 87394-1-RR
Non-treated HeLa cells and paclitaxel treated HeLa cells were subjected to SDS PAGE followed by western blot with 87394-1-RR (Phospho-NPM1 (Thr199) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
SEC-HPLC of 87394-1-RR
The purity of Human Phospho-NPM1 (Thr199) Recombinant monoclonal antibody (Cat. No. 87394-1-RR) was greater than 95% as determined by SEC-HPLC.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
FC experiment of Jurkat using 87394-1-RR
1x10^6 Jurkat cells untreated (dashed lines) or treated with paclitaxel which intracellularly stained with 0.13 ug Phospho-NPM1 (Thr199) Recombinant monoclonal antibody (87394-1-RR, Clone:252639G7) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.13 ug Isotype Control (blue). Cells were fixed and permeabilized with Flow Cytometry Phosphorylated Protein Fixation/Permeabilization Kit (PF00026).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
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1x10^6 Jurkat cells untreated (dashed lines) or treated with paclitaxel which intracellularly stained with 0.13 ug Phospho-NPM1 (Thr199) Recombinant monoclonal antibody (87394-1-RR, Clone:252639G7) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.13 ug Isotype Control (blue). Cells were fixed and permeabilized with Flow Cytometry Phosphorylated Protein Fixation/Permeabilization Kit (PF00026).

Overview

Tested Applications
Product Information
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MSDS SDS

Tested Applications

Positive WB detected in	paclitaxel treated HeLa cells
Positive FC (Intra) detected in	paclitaxel treated Jurkat cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

87394-1-RR targets Phospho-NPM1 (Thr199) in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	Peptide Predict reactive species
Full Name	nucleophosmin (nucleolar phosphoprotein B23, numatrin)
Calculated Molecular Weight	33 kDa
Observed Molecular Weight	40 kDa
GenBank Accession Number	NM_002520
Gene Symbol	NPM1
Gene ID (NCBI)	4869
RRID	AB_3745512
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P06748
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Nucleophosmin (NPM1,B23) is a putative ribosome assembly factor with a high affinity for peptides containing nuclear localization signals (NLSs). Phospho-NPM1 (Thr199) is a phosphorylated form of nucleophosmin (NPM1) at threonine 199, a residue that plays a critical role in cell cycle regulation and cancer development. Indeed, phosphorylation by CDK2/cyclin E on Thr199 residue during G1 phase is critical for the NPM1 dissociation from the centrosome to initiate its duplication and the pre-mRNA processing. (PMID: 26993766)