HGS Recombinant monoclonal antibody

HGS Uni-rAb^® Recombinant Antibody for WB, IF/ICC, IP, ELISA

Cat No. 87420-1-RR

Clone No.252813G1

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, ELISA

Synonyms

Hepatocyte growth factor-regulated tyrosine kinase substrate, Hrs, Protein pp110, ZFYVE8

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488

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Filter: AllWBIPIF/ICCAffinity

WB analysis using 87420-1-RR
Various lysates were subjected to SDS PAGE followed by western blot with 87420-1-RR (HGS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IP experiment of mouse brain using 87420-1-RR
IP result of anti-HGS (IP:87420-1-RR, 4ug; Detection:87420-1-RR 1:3000) with mouse brain tissue lysate 1960 ug.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of HeLa using 87420-1-RR
Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using HGS antibody (87420-1-RR, Clone: 252813G1 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of HepG2 using 87420-1-RR
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using HGS antibody (87420-1-RR, Clone: 252813G1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of Saos-2 using 87420-1-RR
Immunofluorescent analysis of (4% PFA) fixed Saos-2 cells using HGS antibody (87420-1-RR, Clone: 252813G1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
Affinity of 87420-1-RR
Biolayer interferometry (BLl) kinetic assays of 87420-1-RR against Human HGS were performed. The affinity constant is 5.10 nM.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Overview

Tested Applications
Product Information
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	HeLa cells, HepG2 cells, A549 cells, K-562 cells, Jurkat cells, Neuro-2a cells, rat brain tissue
Positive IP detected in	mouse brain tissue
Positive IF/ICC detected in	Saos-2 cells, HeLa cells, HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

87420-1-RR targets HGS in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Ag0589 Product name: Recombinant human HGS protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 186-387 aa of BC003565 Sequence: GQIFCGKCSSKYSTIPKFGIEKEVRVCEPCYEQLNRKAEGKATSTTELPPEYLTSPLSQQSQLPPKRDETALQEEEELQLALALSQSEAEEKERLRQKSTYTSYPKAEPMPSASSAPPASSLYSSPVNSSAPLAEDIDPELARYLNRNYWEKKQEEARKSPTPSAPVPLTEPAAQPGEGHAAPTNVVENPLPETDSQPIPPS Predict reactive species
Full Name	hepatocyte growth factor-regulated tyrosine kinase substrate
Calculated Molecular Weight	86 kDa
Observed Molecular Weight	110 kDa
GenBank Accession Number	BC003565
Gene Symbol	HGS
Gene ID (NCBI)	9146
RRID	AB_3745532
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	O14964
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Hepatocyte growth factor-regulated tyrosine kinase substrate (HGS, synonyms: HRS, ZFYVE8) is a 110 to 115-kDa zinc finger phosphotyrosine protein inducible by stimulation with interleukin 2 (IL-2), granulocyte-macrophage colony-stimulating factor (GM-CSF) as well as hepatocyte growth factor (HGF), and is associated with signal-transducing adaptor molecule (STAM). HGS suppresses DNA synthesis upon stimulation with IL-2 and GM-CSF, counteracting STAM's function, which is critical for cell growth signaling mediated by the cytokines. HGS also interacts with the neurofibromatosis 2 tumor suppressor protein Schwannomin/merlin. The growth suppression activity of schwannoma/merlin requires HGS. The binding of schwannoma/merlin to HGS facilitates its ability to function as a tumor suppressor, probably by inhibiting STAT activation.

Protocols

Product Specific Protocols
IF protocol for HGS antibody 87420-1-RR	Download protocol
IP protocol for HGS antibody 87420-1-RR	Download protocol
WB protocol for HGS antibody 87420-1-RR	Download protocol