Tested Applications
| Positive WB detected in | HeLa cells, HL-60 cells, K-562 cells, THP-1 cells, PC-12 cells, NIH/3T3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
87483-1-RR targets TXNIP in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag33838 Product name: Recombinant human TXNIP protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-391 aa of BC093702 Sequence: MVMFKKIKSFEVVFNDPEKVYGSGEKVAGRVIVEVCEVTRVKAVRILACGVAKVLWMQGSQQCKQTSEYLRYEDTLLLEDQPTGENEMVIMRPGNKYEYKFGFELPQGPLGTSFKGKYGCVDYWVKAFLDRPSQPTQETKKNFEVVDLVDVNTPDLMAPVSAKKEKKVSCMFIPDGRVSVSARIDRKGFCEGDEISIHADFENTCSRIVVPKAAIVARHTYLANGQTKVLTQKLSSVRGNHIISGTCASWRGKSLRVQKIRPSILGCNILRVEYSLLIYVSVPGSKKVILDLPLVIGSRSGLSSRTSSMASRTSSEMSWVDLNIPDTPEAPPCYMDVIPEDHRLESPTTPLLDDMDGSQDSPIFMYAPEFKFMPPPTYTEVDPCILNNNVQ Predict reactive species |
| Full Name | thioredoxin interacting protein |
| Calculated Molecular Weight | 391 aa, 44 kDa |
| Observed Molecular Weight | 44-55 kDa |
| GenBank Accession Number | BC093702 |
| Gene Symbol | TXNIP |
| Gene ID (NCBI) | 10628 |
| RRID | AB_3745575 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9H3M7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TXNIP, also known as VDUP-1 or TBP-2, belongs to alpha-arrestin protein family and is perhaps the only family member known to bind thioredoxin (TRX). TXNIP was induced by Vitamin D3, but not induced by another monocyte or macrophage differentiation inducer: phorbol 12-myristate 13-acetate (PMA). TXNIP bound catalytic active center of thioredoxin (TRX), which protected cells against oxidative stress. TXNIP was found to be a negative regulator of thioredoxin activity and inducer of the intracellular level of reactive oxygen species (ROS). TXNIP plays an important role in a wide variety of biological functions, such as the regulation of cell death, growth, differentiation, and energy metabolism.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TXNIP antibody 87483-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
