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GPR183 Recombinant monoclonal antibody (87492-4-RR)

GPR183 Recombinant monoclonal antibody

GPR183 Uni-rAb^® Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 87492-4-RR

Clone No.260283A6

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

EBI2, GPR183/EBI2, CDw381, EBV-induced G-protein coupled receptor 2, Epstein-Barr virus-induced G-protein coupled receptor 2

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

SKU:

-/ -

Freight/Packing: -

Quantity

- +

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Filter: AllWBIF/ICC

WB analysis using 87492-4-RR
Various lysates were subjected to SDS PAGE followed by western blot with 87492-4-RR (GPR183 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of Raji using 87492-4-RR
Immunofluorescent analysis of (4% PFA) fixed Raji cells using GPR183 antibody (87492-4-RR, Clone: 260283A6 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of Daudi using 87492-4-RR
Immunofluorescent analysis of (4% PFA) fixed Daudi cells using GPR183 antibody (87492-4-RR, Clone: 260283A6 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Overview

Tested Applications
Product Information
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	Raji cells, Daudi cells, Fetal human brain tissue
Positive IF/ICC detected in	Raji cells, Daudi cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

87492-4-RR targets GPR183 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	Peptide Predict reactive species
Full Name	G protein-coupled receptor 183
Calculated Molecular Weight	361 aa, 41 kDa
Observed Molecular Weight	50-55 kDa
GenBank Accession Number	BC020752
Gene Symbol	EBI2
Gene ID (NCBI)	1880
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P32249
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

G-protein-coupled receptor 183, also known as Epstein-Barr virus-induced gene 2 (EBI2), was initially identified in B cells and is the most upregulated gene after infection with Epstein-Barr virus (PMID:37353188). GPR183 is known to play a key role in the development of antibody responses in secondary lymphoid organs (PMID:23473835).