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Tested Applications
| Positive WB detected in | human milk |
| Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human tonsillitis tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF)-P | IF-P : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
87606-3-RR targets Human IgA1 in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Eg9190 Product name: Recombinant human IgA1-IgK protein Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS Tag: C-6*HIS Sequence: \ Predict reactive species |
| Full Name | immunoglobulin heavy constant alpha 1 |
| Observed Molecular Weight | 50-70 kDa |
| Gene Symbol | Human IgA Heavy Chain |
| Gene ID (NCBI) | 3493 |
| RRID | AB_3745646 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Human IgA1 is one of the two subclasses of Immunoglobulin A (IgA), the most abundantly produced antibody in the human body . While IgA is the predominant antibody class at mucosal surfaces (such as the gut, respiratory, and urogenital tracts), IgA1 is the dominant subclass in the blood (serum), comprising approximately 85-90% of total serum IgA .
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Human IgA1 antibody 87606-3-RR | Download protocol |
| IHC protocol for Human IgA1 antibody 87606-3-RR | Download protocol |
| WB protocol for Human IgA1 antibody 87606-3-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
