Tested Applications
| Positive WB detected in | HeLa cells, HepG2 cells, K-562 cells, MCF-7 cells, Jurkat cells, RAW 264.7 cells, PC-12 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
87643-2-RR targets GTF2B in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag9547 Product name: Recombinant human GTF2B protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-316 aa of BC020597 Sequence: MASTSRLDALPRVTCPNHPDAILVEDYRAGDMICPECGLVVGDRVIDVGSEWRTFSNDKATKDPSRVGDSQNPLLSDGDLSTMIGKGTGAASFDEFGNSKYQNRRTMSSSDRAMMNAFKEITTMADRINLPRNIVDRTNNLFKQVYEQKSLKGRANDAIASACLYIACRQEGVPRTFKEICAVSRISKKEIGRCFKLILKALETSVDLITTGDFMSRFCSNLCLPKQVQMAATHIARKAVELDLVPGRSPISVAAAAIYMASQASAEKRTQKEIGDIAGVADVTIRQSYRLIYPRAPDLFPTDFKFDTPVDKLPQL Predict reactive species |
| Full Name | general transcription factor IIB |
| Calculated Molecular Weight | 316 aa, 35 kDa |
| Observed Molecular Weight | 30-35 kDa |
| GenBank Accession Number | BC020597 |
| Gene Symbol | GTF2B |
| Gene ID (NCBI) | 2959 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q00403 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
GTF2B encodes transcription factor IIB (TFIIB), one of the ubiquitous general transcription factors essential for transcription initiation by RNA polymerase II (Pol II). Localizing to the nucleus, TFIIB forms a complex with transcription factors IID and IIA and serves as a critical bridge between TFIID-the factor that initially recognizes the promoter-and the Pol II machinery, thereby enabling pre-initiation complex (PIC) assembly and Pol II recruitment to promoter DNA. Beyond this core architectural role, TFIIB is subject to regulatory acetylation and autoacetylation, and emerging evidence links its dysregulation to pathological states, including potential contributions to proliferation in hepatocellular carcinoma and tumor phenotypes in growth hormone-secreting pituitary adenomas.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for GTF2B antibody 87643-2-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
