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MEF2A Recombinant monoclonal antibody, PBS Only (Detector) (87660-2-PBS)

MEF2A Recombinant monoclonal antibody, PBS Only (Detector)

Name: MEF2A Recombinant monoclonal antibody, PBS Only (Detector), 100ug
Brand: Proteintech
SKU: 87660-2-PBS-100UG

MEF2A Uni-rAb^® Recombinant Antibody for WB, IF/ICC, Sandwich ELISA, Indirect ELISA

Cat No. 87660-2-PBS

Clone No.253110B3

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Sandwich ELISA, Indirect ELISA

Synonyms

ADCAD1, MEF2, myocyte enhancer factor 2A, Myocyte-specific enhancer factor 2A, RSRFC4

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

SKU:

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Freight/Packing: -

Quantity

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Filter: AllWBSandwich ELISA sample testSandwich ELISAIF/ICC

WB analysis using 87660-2-RR (same clone as 87660-2-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 87660-2-RR (MEF2A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87660-2-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of THP-1 using 87660-2-RR (same clone as 87660-2-PBS)
WB result of MEF2A antibody (87660-2-RR; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-MEF2A transfected THP-1 cells. This data was developed using the same antibody clone with 87660-2-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
Sandwich ELISA sample test of MP03216-1
The mean MEF2A concentration was determined to be 4.99 ng/mL in HeLa cell extract based on a 1.20 mg/mL extract load.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
Sandwich ELISA sample test of MP03216-1
Plasma of six individual healthy human donors was measured. The MEF2A concentration of detected samples was determined to be 0.30 ng/mL with a range of 0.07-0.78 ng/mL.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
Sandwich ELISA standard curve of MP03216-1
Sandwich ELISA standard curve of MP03216-1, Human MEF2A Recombinant Matched Antibody Pair - PBS only. 87660-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag41519. 87660-2-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of HeLa using 87660-2-RR (same clone as 87660-2-PBS)
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MEF2A antibody (87660-2-RR, Clone: 253110B3 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 87660-2-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

87660-2-PBS targets MEF2A as part of a matched antibody pair:

MP03216-1: 87660-3-PBS capture and 87660-2-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Ag41519 Product name: Recombinant human MEF2A protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 150-499 aa of BC013437 Sequence: SPNALSYTNPGSSLVSPSLAASSTLTDSSMLSPPQTTLHRNVSPGAPQRPPSTGNAGGMLSTTDLTVPNGAGSSPVGNGFVNSRASPNLIGATGANSLGKVMPTKSPPPPGGGNLGMNSRKPDLRVVIPPSSKGMMPPLNTQRISSSQATQPLATPVVSVTTPSLPPQGLVYSAMPTAYNTDYSLTSADLSALQGFNSPGMLSLGQVSAWQQHHLGQAALSSLVAGGQLSQGSNLSINTNQNISIKSEPISPPRDRMTPSGFQQQQQQQQQQQPPPPPQPQPQPPQPQPRQEMGRSPVDSLSSSSSSYDGSDREDPRGDFHSPIVLGRPPNTEDRESPSVKRMRMDAWVT Predict reactive species
Full Name	myocyte enhancer factor 2A
Calculated Molecular Weight	499 aa, 54 kDa
Observed Molecular Weight	50-70kDa
GenBank Accession Number	BC013437
Gene Symbol	MEF2A
Gene ID (NCBI)	4205
RRID	AB_3745680
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q02078
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

MEF2A belongs to a family of DNA binding regulatory proteins. Each of these proteins which belong to the myocyte-specific enhancer factor-2 (MEF2) family binds to the MEF2 target DNA sequence present in the regulatory regions of many, if not all, muscle-specific genes. MEF2A plays diverse roles in the control of cell growth, survival and apoptosis via p38 MAPK signaling in muscle-specific and/or growth factor-related transcription. MEF2A also has key roles in cardiac and skeletal muscle development. MEF2A is especially abundant in granule neurons of the cerebellar cortex throughout the period of synaptogenesis. This antibody can detect two bands around 60 kDa to 70 kDa, the larger band may correspond to its post-translational modifications.(PMID: 15483225,16484498, 23486945 )