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Sandwich ELISA sample test of MP03175-1
HUVEC human umbilical vein endothelial cells were cultured in EGM-2. Aliquots of the cell culture supernatants were removed, assayed for levels of DKK3, and measured 12.24 ng/mL
Sandwich ELISA sample test of MP03175-1
Plasma of six individual healthy human donors was measured. The human DKK3 concentration of detected samples was determined to be 63.82 ng/mL with a range of 52.74-78.16 ng/mL.
Sandwich ELISA standard curve of MP03175-1
Sandwich ELISA standard curve of MP03175-1, Human DKK3 Recombinant Matched Antibody Pair - PBS only. 87713-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg6731. 87713-2-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL
Cytometric bead array sample test of MP03175-1
The mean DKK3 concentration was determined to be 43.0 ng/mL in human serum 1, 41.0 ng/mL in human serum 2.
Cytometric bead array standard curve of MP03175-1
Cytometric bead array standard curve of MP03175-1, DKK3 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 87713-3-PBS. Detection antibody: 87713-2-PBS. Standard: Eg6731. Range: 0.156-20 ng/mL.

DKK3 Recombinant monoclonal antibody, PBS Only (Detector)

Name: DKK3 Recombinant monoclonal antibody, PBS Only (Detector)
Brand: Proteintech
SKU: 87713-2-PBS

DKK3 Uni-rAb^® Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA

Cat No. 87713-2-PBS

Clone No.253091F2

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA

Synonyms

Formulation: PBS Only

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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Product Information

87713-2-PBS targets DKK3 as part of a matched antibody pair:

MP03175-1: 87713-3-PBS capture and 87713-2-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Eg6731 Product name: Recombinant human DKK3 protein Source: mammalian cells-derived, pHZ-KIsec-C-6HIS Tag: C-6HIS Domain: 22-350 aa of NM_015881.5, NM_013253.4, NM_001018057.1 Sequence: APAPTATSAPVKPGPALSYPQEEATLNEMFREVEELMEDTQHKLRSAVEEMEAEEAAAKASSEVNLANLPPSYHNETNTDTKVGNNTIHVHREIHKITNNQTGQMVFSETVITSVGDEEGRRSHECIIDEDCGPSMYCQFASFQYTCQPCRGQRMLCTRDSECCGDQLCVWGHCTKMATRGSNGTICDNQRDCQPGLCCAFQRGLLFPVCTPLPVEGELCHDPASRLLDLITWELEPDGALDRCPCASGLLCQPHSHSLVYVCKPTFVGSRDQDGEILLPREVPDEYEVGSFMEEVRQELEDLERSLTEEMALREPAAAAAALLGGEEI Predict reactive species
Full Name	dickkopf homolog 3 (Xenopus laevis)
Calculated Molecular Weight	38 kDa
GenBank Accession Number	NM_015881.5, NM_013253.4, NM_001018057.1
Gene Symbol	DKK3
Gene ID (NCBI)	27122
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q9UBP4
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.