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AP1B1 Recombinant monoclonal antibody, PBS Only (Detector) (87859-2-PBS)

Filter: AllWBIPIF/ICCCytometric bead array sample testCytometric bead arrayAffinity

WB analysis of HEK-293 using 87859-2-RR (same clone as 87859-2-PBS)
WB result of AP1B1 antibody (87859-2-RR; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AP1B1 transfected HEK-293 cells. This data was developed using the same antibody clone with 87859-2-PBS in a different storage buffer formulation.
WB analysis using 87859-2-RR (same clone as 87859-2-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 87859-2-RR (AP1B1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87859-2-PBS in a different storage buffer formulation.
IP experiment of HEK-293 using 87859-2-RR (same clone as 87859-2-PBS)
IP result of anti-AP1B1 (IP:87859-2-RR, 4ug; Detection:87859-2-RR 1:3000) with HEK-293 cells lysate 1560 ug. This data was developed using the same antibody clone with 87859-2-PBS in a different storage buffer formulation.
IF Staining of HepG2 using 87859-2-RR (same clone as 87859-2-PBS)
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using AP1B1 antibody (87859-2-RR, Clone: 253524D8 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 87859-2-PBS in a different storage buffer formulation.
Cytometric bead array sample test of MP03244-1
The mean AP1B1 concentration was determined to be 118.8 ng/mL in A431 cell extract based on a 1.2 mg/mL extract load, 163.8 ng/mL in HEK-293 cell extract based on a 1.5 mg/mL extract load, 80.2 ng/mL in A2780 cell extract based on a 1.2 mg/mL extract load.
Cytometric bead array standard curve of MP03244-1
Cytometric bead array standard curve of MP03244-1, AP1B1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 87859-3-PBS. Detection antibody: 87859-2-PBS. Standard: Ag10417. Range: 0.312-40 ng/mL.
Affinity of 87859-2-RR
Biolayer interferometry (BLI) kinetic assays of 87859-2-RR against Human AP1B1 were performed. The affinity constant is 0.356 nM.

AP1B1 Recombinant monoclonal antibody, PBS Only (Detector)

Name: AP1B1 Recombinant monoclonal antibody, PBS Only (Detector)
Brand: Proteintech
SKU: 87859-2-PBS

AP1B1 Uni-rAb^® Recombinant Antibody for WB, IF/ICC, IP, Cytometric bead array, Indirect ELISA

Cat No. 87859-2-PBS

Clone No.253524D8

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, IP, Cytometric bead array, Indirect ELISA

Synonyms

Adaptor protein complex AP-1 subunit beta-1, Adaptor-related protein complex 1 subunit beta-1, ADTB1, AP 1 complex subunit beta 1, AP-1 complex subunit beta-1

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

87859-2-PBS targets AP1B1 as part of a matched antibody pair:

MP03244-1: 87859-3-PBS capture and 87859-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Ag10417 Product name: Recombinant human AP1B1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 671-919 aa of BC046242 Sequence: NFVAPPTAAVPANLGAPIGSGLSDLFDLTSGVGTLSGSYVAPKAVGSISMDLQLTNKALQVMTDFAIQFNRNSFGLAPAAPLQVHAPLSPNQTVEISLPLSTVGSVMKMEPLNNLQVAVKNNIDVFYFSTLYPLHILFVEDGKMDRQMFLATWKDIPNENEAQFQIRDCPLNAEAASSKLQSSNIFTVAKRNVEGQDMLYQSLKLTNGIWVLAELRIQPGNPSCTLSLKCRAPEVSQHVYQAYETILKN Predict reactive species
Full Name	adaptor-related protein complex 1, beta 1 subunit
Calculated Molecular Weight	919 aa, 101 kDa
Observed Molecular Weight	101 kDa
GenBank Accession Number	BC046242
Gene Symbol	AP1B1
Gene ID (NCBI)	162
RRID	AB_3745793
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q10567
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

AP1B1 is a subunit of the adaptor complex AP-1 and is a member of the adaptin protein family. Adaptor protein (AP) complexes are cytosolic heterotetramers that mediate the sorting of membrane proteins in the secretory and endocytic pathways. AP-1 is found at the cytoplasmic face of coated vesicles located at the Golgi complex, where it mediates both the recruitment of clathrin to the membrane and the recognition of sorting signals within the cytosolic tails of transmembrane receptors.