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Phospho-ALK (Tyr1278) Recombinant monoclonal antibody (87874-1-RR)

Phospho-ALK (Tyr1278) Recombinant monoclonal antibody

Phospho-ALK (Tyr1278) Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 87874-1-RR

Clone No.253445D5

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

ALK (Tyr1278), p-ALK (Tyr1278), pALK Y1278, Phospho-ALK, Phospho-NPM-ALK

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

SKU:

-/ -

Freight/Packing: -

Quantity

- +

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WB analysis of Karpas-299 using 87874-1-RR
Non-treated and pervanadate treated Karpas-299 cells were subjected to SDS PAGE followed by western blot with 87874-1-RR (Phospho-ALK (Tyr1278) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Vinculin (86930-1-RR) antibody as loading control.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Overview

Tested Applications
Product Information
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in

pervanadate treated Karpas-299 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

87874-1-RR targets Phospho-ALK (Tyr1278) in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	Peptide Predict reactive species
Full Name	anaplastic lymphoma receptor tyrosine kinase
Calculated Molecular Weight	176 kDa
Observed Molecular Weight	80 kDa, 300 kDa
GenBank Accession Number	NM_004304
Gene Symbol	ALK
Gene ID (NCBI)	238
RRID	AB_3745801
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q9UM73
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Anaplastic lymphoma kinase was first described in 1994 as the NPM-ALK fusion protein that is expressed in the majority of anaplastic large-cell lymphomas. About 2/3rds of anaplastic large-cell lymphomas possess a balanced chromosomal translocation in which the entire nucle-ophosmin (NPM) gene on chromosome 5 is fused to the 3' portion of the ALK gene (including the entire intracellular segment with its protein kinase domain) on chromosome 2. This oncogenic ALK protein kinase is a chimeric protein created by a translocation between chromosomes(2;5)(p23;q35) that generates the NPM-ALK fusion protein. Phospho-ALK (Tyr1278) is a hallmark of ALK-driven malignancies. (PMID: 30400214)