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MGA Recombinant monoclonal antibody, PBS Only (Capture) (87932-2-PBS)

MGA Recombinant monoclonal antibody, PBS Only (Capture)

Name: MGA Recombinant monoclonal antibody, PBS Only (Capture), 100ug
Brand: Proteintech
SKU: 87932-2-PBS-100UG

MGA Uni-rAb^® Recombinant Antibody for Sandwich ELISA, Indirect ELISA

Cat No. 87932-2-PBS

Clone No.253246G10

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Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Sandwich ELISA, Indirect ELISA

Synonyms

Formulation: PBS Only

PBS Only

Conjugate: Unconjugated

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Filter: AllSandwich ELISA sample testSandwich ELISA

Sandwich ELISA sample test of MP03262-1
The mean MGAM concentration was determined to be 439.00 ng/mL in HL-60 cell extract based on a 1.5 mg/mL extract load, 461.37 ng/mL in COLO 320 cell extract based on a 1.2 mg/mL extract load, 144.04 ng/mL in A2780 cell extract based on a 1.2 mg/mL extract load, 173.49 ng/mL in Raji cell extract based on a 1.2 mg/mL extract load and 118.72 ng/mL in Ramos cell extract based on a 1.2 mg/mL extract load.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
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The mean MGAM concentration was determined to be 439.00 ng/mL in HL-60 cell extract based on a 1.5 mg/mL extract load, 461.37 ng/mL in COLO 320 cell extract based on a 1.2 mg/mL extract load, 144.04 ng/mL in A2780 cell extract based on a 1.2 mg/mL extract load, 173.49 ng/mL in Raji cell extract based on a 1.2 mg/mL extract load and 118.72 ng/mL in Ramos cell extract based on a 1.2 mg/mL extract load.
Sandwich ELISA standard curve of MP03262-1
Sandwich ELISA standard curve of MP03262-1, Human MGAM Recombinant Matched Antibody Pair - PBS only. 87932-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg6430. 87932-1-PBS was HRP conjugated as the detection antibody. Range: 0.625-40 ng/mL
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Overview

Tested Applications
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87932-2-PBS targets MGA as part of a matched antibody pair:

MP03262-1: 87932-2-PBS capture and 87932-1-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Eg6430 Product name: Recombinant human MGA protein Source: mammalian cells-derived, V37 Tag: C-rFc Domain: 85-960 aa of NM_001365693.1 Sequence: PVSAECPVVNELERINCIPDQPPTKATCDQRGCCWNPQGAVSVPWCYYSKNHSYHVEGNLVNTNAGFTARLKNLPSSPVFGSNVDNVLLTAEYQTSNRFHFKLTDQTNNRFEVPHEHVQSFSGNAAASLTYQVEISRQPFSIKVTRRSNNRVLFDSSIGPLLFADQFLQLSTRLPSTNVYGLGEHVHQQYRHDMNWKTWPIFNRDTTPNGNGTNLYGAQTFFLCLEDASGLSFGVFLMNSNAMEVVLQPAPAITYRTIGGILDFYVFLGNTPEQVVQEYLELIGRPALPSYWALGFHLSRYEYGTLDNMREVVERNRAAQLPYDVQHADIDYMDERRDFTYDSVDFKGFPEFVNELHNNGQKLVIIVDPAISNNSSSSKPYGPYDRGSDMKIWVNSSDGVTPLIGEVWPGQTVFPDYTNPNCAVWWTKEFELFHNQVEFDGIWIDMNEVSNFVDGSVSGCSTNNLNNPPFTPRILDGYLFCKTLCMDAVQHWGKQYDIHNLYGYSMAVATAEAAKTVFPNKRSFILTRSTFAGSGKFAAHWLGDNTATWDDLRWSIPGVLEFNLFGIPMVGPDICGFALDTPEELCRRWMQLGAFYPFSRNHNGQGYKDQDPASFGADSLLLNSSRHYLNIRYTLLPYLYTLFFRAHSRGDTVARPLLHEFYEDNSTWDVHQQFLWGPGLLITPVLDEGAEKVMAYVPDAVWYDYETGSQVRWRKQKVEMELPGDKIGLHLRGGYIFPTQQPNTTTLASRKNPLGLIIALDENKEAKGELFWDNGETKDTVANKVYLLCEFSVTQNRLEVNISQSTYKDPNNLAFNEIKILGTEEPSNVTVKHNGVPSQTSPTVTYDSNLKVAIITDIDLLLGEAYTVEWSIKIRD Predict reactive species
Full Name	maltase-glucoamylase (alpha-glucosidase)
Calculated Molecular Weight	312 kDa
GenBank Accession Number	NM_001365693.1
Gene Symbol	MGAM
Gene ID (NCBI)	8972
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	O43451-2
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

MGA Recombinant monoclonal antibody, PBS Only (Capture)

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Sandwich ELISA sample test of MP03262-1

Sandwich ELISA standard curve of MP03262-1

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