PCK2 Recombinant monoclonal antibody

PCK2 Uni-rAb^® Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 88027-1-RR

Clone No.260096E12

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IF/ICC, ELISA

Synonyms

EC:4.1.1.32, mtPCK2, PEPCK2, PEPCK-M, Phosphoenolpyruvate carboxykinase 2, mitochondrial

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

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Filter: AllWBIF/ICC

WB analysis using 88027-1-RR
Various lysates were subjected to SDS PAGE followed by western blot with 88027-1-RR (PCK2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of HepG2 using 88027-1-RR
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PCK2 antibody (88027-1-RR, Clone: 260096E12 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Overview

Tested Applications
Product Information
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	LNCaP cells, HepG2 cells, K-562 cells, Neuro-2a cells, HSC-T6 cells
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunofluorescence (IF)/ICC	IF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

88027-1-RR targets PCK2 in WB, IF/ICC, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity	human, rat
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Ag6681 Product name: Recombinant human PCK2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 292-640 aa of BC001454 Sequence: AGKKRYVAAAFPSACGKTNLAMMRPALPGWKVECVGDDIAWMRFDSEGRLRAINPENGFFGVAPGTSATTNPNAMATIQSNTIFTNVAETSDGGVYWEGIDQPLPPGVTVTSWLGKPWKPGDKEPCAHPNSRFCAPARQCPIMDPAWEAPEGVPIDAIIFGGRRPKGVPLVYEAFNWRHGVFVGSAMRSESTAAAEHKGKIIMHDPFAMRPFFGYNFGHYLEHWLSMEGRKGAQLPRIFHVNWFRRDEAGHFLWPGFGENARVLDWICRRLEGEDSARETPIGLVPKEGALDLSGLRAIDTTQLFSLPKDFWEQEVRDIRSYLTEQVNQDLPKEVLAELEALERRVHKM Predict reactive species
Full Name	phosphoenolpyruvate carboxykinase 2 (mitochondrial)
Calculated Molecular Weight	71 kDa
Observed Molecular Weight	66-71 kDa
GenBank Accession Number	BC001454
Gene Symbol	PCK2
Gene ID (NCBI)	5106
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q16822
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PCK2(phosphoenolpyruvate carboxykinase [GTP], mitochondrial) is also named as PEPCK2, PEPCK-M and belongs to the phosphoenolpyruvate carboxykinase [GTP] family. It catalyses the irreversible conversion of oxaloacetate to phosphoenolpyruvate, an important initial step in hepatic gluconeogenesis.Defects in PCK2 are the cause of mitochondrial phosphoenolpyruvate carboxykinase deficiency (M-PEPCKD). It has 2 isoforms produced by alternative splicing with the molecular weight of 71 kDa and 48 kDa. This antibody specifically recognizes PCK2.