IRAK4 Recombinant monoclonal antibody, PBS Only (Capture)

The mean IRAK4 concentration was determined to be 568.41 ng/mL in THP-1 cell extract based on a 1.20 mg/mL extract load, 456.19 ng/mL in HeLa cell extract based on a 1.20 mg/mL extract load, 435.79 ng/mL in SH-SY5Y cell extract based on a 1.20 mg/mL extract load and 403.69 ng/mL in U2OS cell extract based on a 1.20 mg/mL extract load.

The mean IRAK4 concentration was determined to be 1,268.87 ng/mL in Jurkat cell extract based on a 1.20 mg/mL extract load, 871.67 ng/mL in Ramos cell extract based on a 1.50 mg/mL extract load, 584.26 ng/mL in HL-60 cell extract based on a 1.20 mg/mL extract load and 566.48 ng/mL in K-562 cell extract based on a 1.20 mg/mL extract load.

Sandwich ELISA standard curve of MP03420-1, Human IRAK4 Recombinant Matched Antibody Pair - PBS only. 88124-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag12982. 88124-2-PBS was HRP conjugated as the detection antibody. Range: 1.56-100 ng/mL.