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MFI2 Recombinant monoclonal antibody, PBS Only (Capture) (88127-2-PBS)

MFI2 Recombinant monoclonal antibody, PBS Only (Capture)

Name: MFI2 Recombinant monoclonal antibody, PBS Only (Capture), 100ug
Brand: Proteintech
SKU: 88127-2-PBS-100UG

MFI2 Uni-rAb^® Recombinant Antibody for WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA

Cat No. 88127-2-PBS

Clone No.260392B10

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA

Synonyms

Melanoma-associated antigen p97, Melanotransferrin, MELTF

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

SKU:

-/ -

Freight/Packing: -

Quantity

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Filter: AllWBSandwich ELISA sample testSandwich ELISACytometric bead array sample testCytometric bead array

WB analysis using 88127-2-RR (same clone as 88127-2-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 88127-2-RR (MELTF/CD228 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 88127-2-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
Sandwich ELISA sample test of MP03370-1
The mean MFI2 concentration was determined to be 102.73 ng/mL in HEK-293 cell extract based on a 1.50 mg/mL extract load, 8.59 ng/mL in HeLa cell extract based on a 1.20 mg/mL extract load and 1.26 ng/mL in HL-60 cell extract based on a 1.20 mg/mL extract load.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
Sandwich ELISA standard curve of MP03370-1
Sandwich ELISA standard curve of MP03370-1, Human MFI2 Recombinant Matched Antibody Pair - PBS only. 88127-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg9197. 88127-3-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
Cytometric bead array sample test of MP03370-1
The mean MFI2 concentration was determined to be 42.3 ng/mL in HEK-293 cell extract based on a 1.2 mg/mL extract load, 3.2 ng/mL in HeLa cell extract based on a 1.2 mg/mL extract load, 0.5 ng/mL in Raji cell extract based on a 1.5 mg/mL extract load.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
Cytometric bead array standard curve of MP03370-1
Cytometric bead array standard curve of MP03370-1, MFI2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 88127-2-PBS. Detection antibody: 88127-3-PBS. Standard: Eg9197. Range: 0.312-40 ng/mL.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

88127-2-PBS targets MFI2 as part of a matched antibody pair:

MP03370-1: 88127-2-PBS capture and 88127-3-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Eg9197 Product name: Recombinant human MELTF/CD228 protein Source: mammalian cells-derived, V37 Tag: C-rFc Domain: 20-709 aa of NM_005929.6 Sequence: GMEVRWCATSDPEQHKCGNMSEAFREAGIQPSLLCVRGTSADHCVQLIAAQEADAITLDGGAIYEAGKEHGLKPVVGEVYDQEVGTSYYAVAVVRRSSHVTIDTLKGVKSCHTGINRTVGWNVPVGYLVESGRLSVMGCDVLKAVSDYFGGSCVPGAGETSYSESLCRLCRGDSSGEGVCDKSPLERYYDYSGAFRCLAEGAGDVAFVKHSTVLENTDGKTLPSWGQALLSQDFELLCRDGSRADVTEWRQCHLARVPAHAVVVRADTDGGLIFRLLNEGQRLFSHEGSSFQMFSSEAYGQKDLLFKDSTSELVPIATQTYEAWLGHEYLHAMKGLLCDPNRLPPYLRWCVLSTPEIQKCGDMAVAFRRQRLKPEIQCVSAKSPQHCMERIQAEQVDAVTLSGEDIYTAGKTYGLVPAAGEHYAPEDSSNSYYVVAVVRRDSSHAFTLDELRGKRSCHAGFGSPAGWDVPVGALIQRGFIRPKDCDVLTAVSEFFNASCVPVNNPKNYPSSLCALCVGDEQGRNKCVGNSQERYYGYRGAFRCLVENAGDVAFVRHTTVFDNTNGHNSEPWAAELRSEDYELLCPNGARAEVSQFAACNLAQIPPHAVMVRPDTNIFTVYGLLDKAQDLFGDDHNKNGFKMFDSSNYHGQDLLFKDATVRAVPVGEKTTYRGWLGLDYVAALEGMSSQQC Predict reactive species
Full Name	antigen p97 (melanoma associated) identified by monoclonal antibodies 133.2 and 96.5
Calculated Molecular Weight	80 kDa
Observed Molecular Weight	82 kDa
GenBank Accession Number	NM_005929.6
Gene Symbol	MFI2
Gene ID (NCBI)	4241
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P08582-1
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

MFI2 (Melanotransferrin/Mtf/TRMF), also known as p97 or CD228, is an iron-binding glycoprotein belonging to the transferrin family. Alternative RNA splicing yields two forms of different length transcripts, one (the longer) bound to the membrane (mMFI2) and the other (the shorter) secreted to the peripheral environment (sMFI2) such as blood and cell supernatant. MFI2 is upregulated in tumor tissues and has been identified as a serological marker of colorectal cancer (PMID: 25216327).