Home
Products
Primary Antibodies
Recombinant Antibodies
SH3RF1/POSH Recombinant monoclonal antibody (88135-1-RR)

SH3RF1/POSH Recombinant monoclonal antibody

SH3RF1/POSH Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 88135-1-RR

Clone No.260354A12

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

POSH, SH3RF1, E3 ubiquitin-protein ligase SH3RF1, EC:2.3.2.27, KIAA1494

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

SKU:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

Proteintech Guarantee

Filter: AllWB

WB analysis using 88135-1-RR
Various lysates were subjected to SDS PAGE followed by western blot with 88135-1-RR (SH3RF1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis using 88135-1-RR
Various lysates were subjected to SDS PAGE followed by western blot with 88135-1-RR (SH3RF1/POSH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Overview

Tested Applications
Product Information
Protocols

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in

HeLa cells, HEK-293 cells, DU 145 cells, A431 cells, Neuro-2a cells, C6 cells, mouse brain tissue

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

88135-1-RR targets SH3RF1/POSH in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Ag6243 Product name: Recombinant human SH3RF1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 696-1056 aa of BC053671 Sequence: VTMVSPSTAGGPAQKLQGNGVAGSPSVVPAAVVSAAHIQTSPQAKVLLHMTGQMTVNQARNAVRTVAAHNQERPTAAVTPIQVQNAAGLSPASVGLSHHSLASPQPAPLMPGSATHTAAISISRASAPLACAAAAPLTSPSITSASLEAEPSGRIVTVLPGLPTSPDSASSACGNSSATKPDKDSKKEKKGLLKLLSGASTKRKPRVSPPASPTLEVELGSAELPLQGAVGPELPPGGGHGRAGSCPVDGDGPVTTAVAGAALAQDAFHRKASSLDSAVPIAPPPRQACSSLGPVLNESRPVVCERHRVVVSYPPQSEAELELKEGDIVFVHKKREDGWFKGTLQRNGKTGLFPGSFVENI Predict reactive species
Full Name	SH3 domain containing ring finger 1
Calculated Molecular Weight	93 kDa
Observed Molecular Weight	93-100 kDa
GenBank Accession Number	BC053671
Gene Symbol	SH3RF1/POSH
Gene ID (NCBI)	57630
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q7Z6J0
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

POSH (plenty of SH3 domains) is a multidomain GTP-Rac1-interacting protein that acts as a scaffold for JNK activation, leading to apoptosis in neuronal and non-neuronal cells. It consists a RING-finger domain that possesses ubiquitin-ligase activity and four SH3 domains.