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ACD Recombinant monoclonal antibody, PBS Only (Capture) (88168-3-PBS)

Filter: AllWBSandwich ELISA sample testSandwich ELISACytometric bead array sample testCytometric bead array

WB analysis of IMR-32 using 88168-3-RR (same clone as 88168-3-PBS)
IMR-32 cells were subjected to SDS PAGE followed by western blot with 88168-3-RR (ACD antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 88168-3-PBS in a different storage buffer formulation.
WB analysis using 88168-3-RR (same clone as 88168-3-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 88168-3-RR (ACD antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 88168-3-PBS in a different storage buffer formulation.
Sandwich ELISA sample test of MP03378-1
The mean ACD concentration was determined to be 31.68 ng/mL in Jurkat cell extract based on a 1.20 mg/mL extract load, 11.53 ng/mL in HeLa cell extract based on a 1.20 mg/mL extract load, 6.55 ng/mL in Raji cell extract based on a 1.50 mg/mL extract load and 3.19 ng/mL in 786-O cell extract based on a 1.50 mg/mL extract load.
Sandwich ELISA standard curve of MP03378-1
Sandwich ELISA standard curve of MP03378-1, Human ACD Recombinant Matched Antibody Pair - PBS only. 88168-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag23021. 88168-2-PBS was HRP conjugated as the detection antibody. Range: 0.391-25 ng/mL.
Cytometric bead array sample test of MP03378-1
The mean ACD concentration was determined to be 34.1 ng/mL in Jurkat cell extract based on a 1.0 mg/mL extract load, 12.8 ng/mL in HeLa cell extract based on a 1.0 mg/mL extract load, 4.5 ng/mL in Raji cell extract based on a 1.0 mg/mL extract load, 1.2 ng/mL in 786-O cell extract based on a 1.0 mg/mL extract load.
Cytometric bead array standard curve of MP03378-1
Cytometric bead array standard curve of MP03378-1, ACD Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 88168-3-PBS. Detection antibody: 88168-2-PBS. Standard: Ag23021. Range: 0.312-40 ng/mL.

ACD Recombinant monoclonal antibody, PBS Only (Capture)

Name: ACD Recombinant monoclonal antibody, PBS Only (Capture), 100ug
Brand: Proteintech
SKU: 88168-3-PBS-100UG

ACD Uni-rAb^® Recombinant Antibody for WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA

Cat No. 88168-3-PBS

Clone No.260314F11

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA

Synonyms

PIP1, POT1 and TIN2-interacting protein, PTOP, TINT1, TPP1

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

88168-3-PBS targets ACD as part of a matched antibody pair:

MP03378-1: 88168-3-PBS capture and 88168-2-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Ag23021 Product name: Recombinant human ACD protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-351 aa of BC016904 Sequence: MPGRCQSDAAMRVNGPASRAPAGWTSGSLHTGPRAGRPRAQARGVRGRGLLLRPRPAKELPLPRKGGAWAPAGNPGPLHPLGVAVGMAGSGRLVLRPWIRELILGSETPSSPRAGQLLEVLQDAEAAVAGPSHAPDTSDVGATLLVSDGTHSVRCLVTREALDTSDWEEKEFGFRGTEGRLLLLQDCGVHVQVAEGGAPAEFYLQVDRFSLLPTEQPRLRVPGCNQDLDVQKKLYDCLEEHLSESTSSNAGLSLSQLLDEMREDQEHQGALVCLAESCLTLEGPCTAPPVTHWAASRCKATGEAVYTVPSSMLCISENDQLILSSLGPCQRTQGPELPPPDPALQDLSLTL Predict reactive species
Full Name	adrenocortical dysplasia homolog (mouse)
Calculated Molecular Weight	58 kDa
Observed Molecular Weight	60 kDa
GenBank Accession Number	BC016904
Gene Symbol	ACD
Gene ID (NCBI)	65057
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q96AP0
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

ACD (Adrenocortical Dysplasia Homolog), also known as TPP1 or POT1- and TIN2-Organizing Protein, is a core component of the telomeric Shelterin complex. It participates in telomere length maintenance, telomere structural protection, and regulation of genomic stability. Together with POT1, TIN2, TRF1, TRF2, RAP1, and TIN2, ACD assembles into the Shelterin complex, which coats telomeric DNA, suppresses DNA damage response and telomere fusion, and prevents chromosome ends from being recognized as DNA double-strand breaks. Mutations in the ACD gene cause Dyskeratosis Congenita, Hoyeraal-Hreidarsson syndrome, and bone marrow failure syndromes, and are closely associated with telomerase dysfunction.