Tested Applications
| Positive WB detected in | pervanadate treated Jurkat cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
88263-1-RR targets Phospho-SHP1 (Tyr564) in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | protein tyrosine phosphatase, non-receptor type 6 |
| Calculated Molecular Weight | 597 aa, 68 kDa |
| Observed Molecular Weight | 68 kDa |
| GenBank Accession Number | BC002523 |
| Gene Symbol | PTPN6/SHP1 |
| Gene ID (NCBI) | 5777 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P29350 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Phospho-SHP1 (Tyr564) is the activated tyrosine-phosphorylated form of SHP1 (PTPN6), a hematopoietic-specific non-receptor protein tyrosine phosphatase. This C-terminal Tyr564 residue is phosphorylated primarily by Lyn and other Src-family kinases upon cytokine or immune receptor stimulation. The modification triggers intramolecular binding with SHP1's C-SH2 domain, relieving autoinhibition and boosting catalytic phosphatase activity. It targets STAT5, IL-3Rβ and EPOR to restrain proliferative and inflammatory signaling in myeloid and lymphoid cells. Loss of Tyr564 phosphorylation causes persistent STAT5 hyperactivation, driving myeloproliferative neoplasms such as CMML. It acts as a vital negative immune checkpoint, linking its abundance to leukemia progression and autoimmune inflammatory disorders. (PMID: 20858858, 38203502)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Phospho-SHP1 (Tyr564) antibody 88263-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
