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NOC2L Recombinant monoclonal antibody

NOC2L Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 88267-1-RR
Clone No. 260756G2
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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

NIR, NOC2-like protein, Novel INHAT repressor, Nucleolar complex protein 2 homolog, Protein NOC2 homolog

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
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  • Western Blot (WB) analysis of various lysates using NOC2L Recombinant monoclonal antibody (88267-1-RR)

    WB analysis using 88267-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 88267-1-RR (NOC2L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

    This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
  • Western Blot (WB) analysis of various lysates using NOC2L Recombinant monoclonal antibody (88267-1-RR)

    WB analysis using 88267-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 88267-1-RR (NOC2L antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

    This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
  • Immunohistochemistry (IHC) staining of human placenta tissue using NOC2L Recombinant monoclonal antibody (88267-1-RR)

    IHC staining of human placenta using 88267-1-RR

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 88267-1-RR (NOC2L antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using NOC2L Recombinant monoclonal antibody (88267-1-RR)

    IF Staining of U2OS using 88267-1-RR

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using NOC2L antibody (88267-1-RR, Clone: 260756G2 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

    This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
  • Affinity of 88267-1-RR

    Affinity of 88267-1-RR

    Biolayer interferometry (BLI) kinetic assays of 88267-1-RR against Human NOC2L were performed. The affinity constant is 0.413 nM.

    This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
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Tested Applications

Positive WB detected inmouse cerebellum tissue, U2OS cells, rat cerebellum tissue, HL-60 cells, U-251 cells, HeLa cells, HT-1080 cells, PANC-1 cells, MDA-MB-453 cells
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

88267-1-RR targets NOC2L in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag26952

Product name: Recombinant human NOC2L protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-202 aa of BC003555

Sequence: MAAAGSRKRRLAELTVDEFLASGFDSESESESENSPQAETREAREAARSPDKPGGSPSASRRKGRASEHKDQLSRLKDRDPEFYKFLQENDQSLLNFSDSDSSEEEEGPFHSLPDVLEEASEEEDGAEEGEDGDRVPRGLKGKKNSVPVTVAMVERWKQAAKQRLTPKLFHEVVQAFRAAVATTRGDQESAEANKFQVTDSA

Predict reactive species
Full Name nucleolar complex associated 2 homolog (S. cerevisiae)
Calculated Molecular Weight 749 aa, 85 kDa
Observed Molecular Weight 85 kDa
GenBank Accession NumberBC003555
Gene Symbol NOC2L
Gene ID (NCBI) 26155
Conjugate Unconjugated
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ9Y3T9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NOC2L, also known as NIR, is a transcriptional corepressor with inhibitor of histone acetyltransferase (INHAT) activity (PMID: 16322561). It prevents acetylation of all core histones by the histone acetyltransferase EP300/p300. NOC2L interacts with p53, inhibits p53-activated gene expression, and regulates p53-dependent apoptosis (PMID: 16322561). NIR binds to TAp63 and acts as a repressor of TAp63-mediated transactivation of the p21Cip1 gene (PMID: 20123734). NOC2L is ubiquitously expressed throughout embryonic development and adulthood (PMID: 16322561). NOC2L has been reported as a key regulator of skin development. Epidermis-specific ablation of NOC2L impairs epidermal stratification and barrier function (PMID: 29490983).

Protocols

Product Specific Protocols
IF protocol for NOC2L antibody 88267-1-RRDownload protocol
IHC protocol for NOC2L antibody 88267-1-RRDownload protocol
WB protocol for NOC2L antibody 88267-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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