Tested Applications
| Positive WB detected in | mouse brain tissue, rat brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
88290-1-RR targets NECAB2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag2909 Product name: Recombinant human NECAB2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 125-386 aa of BC016979 Sequence: DHMGDYEDVLASLETLNHSVLKAMGYTKKVYEGGSNVDQFVTRFLLKETANQIQSLLSSVESAVEAIEEQTSQLRQNHIKPSHSAAQTWCGSPTPASAPNHKLMAMEQGKTLPSATEDAKEEGLEAQISRLAELIGRLESKALWFDLQQRLSDEDGTNMHLQLVRQEMAVCPEQLSEFLDSLRQYLRGTTGVRNCFHITAVRLSDGFTFVIYEFWETEEAWKRHLQSPLCKAFRHVKVDTLSQPEALSRILVPAAWCTVGRD Predict reactive species |
| Full Name | N-terminal EF-hand calcium binding protein 2 |
| Calculated Molecular Weight | 386 aa, 42 kDa |
| Observed Molecular Weight | 37 kDa |
| GenBank Accession Number | BC016979 |
| Gene Symbol | NECAB2 |
| Gene ID (NCBI) | 54550 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q7Z6G3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
NECAB2 (N-terminal EF-hand calcium-binding protein 2) is a neuronal calcium sensor that functions as a signaling scaffold to modulate G protein-coupled receptor activity. It binds calcium via its N-terminal EF-hand domain and interacts directly with the adenosine A2A receptor (ADORA2A) and metabotropic glutamate receptor 5 (mGluR5), regulating their cell surface expression, internalization, and downstream signaling such as ERK1/2 activation . The protein is predominantly expressed in the brain and spinal cord, where it plays a critical role in pain signaling; its loss in dorsal root ganglia and spinal interneurons significantly accelerates recovery from inflammatory pain hypersensitivity in mice.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for NECAB2 antibody 88290-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
