Product Information
88290-2-PBS targets NECAB2 as part of a matched antibody pair:
MP03437-1: 88290-2-PBS capture and 88290-1-PBS detection (validated in Cytometric bead array)
Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.
This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag2909 Product name: Recombinant human NECAB2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 125-386 aa of BC016979 Sequence: DHMGDYEDVLASLETLNHSVLKAMGYTKKVYEGGSNVDQFVTRFLLKETANQIQSLLSSVESAVEAIEEQTSQLRQNHIKPSHSAAQTWCGSPTPASAPNHKLMAMEQGKTLPSATEDAKEEGLEAQISRLAELIGRLESKALWFDLQQRLSDEDGTNMHLQLVRQEMAVCPEQLSEFLDSLRQYLRGTTGVRNCFHITAVRLSDGFTFVIYEFWETEEAWKRHLQSPLCKAFRHVKVDTLSQPEALSRILVPAAWCTVGRD Predict reactive species |
| Full Name | N-terminal EF-hand calcium binding protein 2 |
| Calculated Molecular Weight | 386 aa, 42 kDa |
| GenBank Accession Number | BC016979 |
| Gene Symbol | NECAB2 |
| Gene ID (NCBI) | 54550 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q7Z6G3 |
| Storage Buffer | PBS only, pH 7.3. |
| Storage Conditions | Store at -80°C. |
Background Information
NECAB2 (N-terminal EF-hand calcium-binding protein 2) is a neuronal calcium sensor that functions as a signaling scaffold to modulate G protein-coupled receptor activity. It binds calcium via its N-terminal EF-hand domain and interacts directly with the adenosine A2A receptor (ADORA2A) and metabotropic glutamate receptor 5 (mGluR5), regulating their cell surface expression, internalization, and downstream signaling such as ERK1/2 activation . The protein is predominantly expressed in the brain and spinal cord, where it plays a critical role in pain signaling; its loss in dorsal root ganglia and spinal interneurons significantly accelerates recovery from inflammatory pain hypersensitivity in mice.
