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PRPF19 Recombinant monoclonal antibody (88331-1-RR)

PRPF19 Recombinant monoclonal antibody

PRPF19 Uni-rAb^® Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 88331-1-RR

Clone No.260837A11

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

EC:2.3.2.27, hPso4, Pre-mRNA-processing factor 19, PRP19, PSO4

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

SKU:

-/ -

Freight/Packing: -

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Filter: AllWBIF/ICC

WB analysis using 88331-1-RR
Various lysates were subjected to SDS PAGE followed by western blot with 88331-1-RR (PRPF19 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of HeLa using 88331-1-RR
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PRPF19 antibody (88331-1-RR, Clone: 260837A11 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Overview

Tested Applications
Product Information
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	HeLa cells, HEK-293 cells, PC-3 cells, Jurkat cells, A431 cells, mouse kidnet tissue, rat kidney tissue
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunofluorescence (IF)/ICC	IF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

88331-1-RR targets PRPF19 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Ag7700 Product name: Recombinant human PRPF19 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 155-504 aa of BC008719 Sequence: GAGEPMDLGELVGMTPEIIQKLQDKATVLTTERKKRGKTVPEELVKPEELSKYRQVASHVGLHSASIPGILALDLCPSDTNKILTGGADKNVVVFDKSSEQILATLKGHTKKVTSVVFHPSQDLVFSASPDATIRIWSVPNASCVQVVRAHESAVTGLSLHATGDYLLSSSDDQYWAFSDIQTGRVLTKVTDETSGCSLTCAQFHPDGLIFGTGTMDSQIKIWDLKERTNVANFPGHSGPITSIAFSENGYYLATAADDSSVKLWDLRKLKNFKTLQLDNNFEVKSLIFDQSGTYLALGGTDVQIYICKQWTEILHFTEHSGLTTGVAFGHHAKFIASTGMDRSLKFYSL Predict reactive species
Full Name	PRP19/PSO4 pre-mRNA processing factor 19 homolog (S. cerevisiae)
Calculated Molecular Weight	55 kDa
Observed Molecular Weight	55 kDa
GenBank Accession Number	BC008719
Gene Symbol	PRPF19
Gene ID (NCBI)	27339
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q9UMS4
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PRPF19 is involved in several processes, including DNA damage checkpoint signaling; cellular protein metabolic process; and mRNA splicing, via spliceosome.