RGS5 Recombinant monoclonal antibody

RGS5 Uni-rAb^® Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 88404-1-RR

Clone No.260952D7

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

Regulator of G-protein signaling 5

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

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Filter: AllWBIF/ICC

WB analysis using 88404-1-RR
Various lysates were subjected to SDS PAGE followed by western blot with 88404-1-RR (RGS5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of NIH/3T3 using 88404-1-RR
Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using RGS5 antibody (88404-1-RR, Clone: 260952D7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Overview

Tested Applications
Product Information
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	SK-N-SH cells, SH-SY5Y cells, HUVEC cells, NIH/3T3 cells, mouse skeletal muscle tissue, rat skeletal muscle tissue
Positive IF/ICC detected in	NIH/3T3 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

88404-1-RR targets RGS5 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Ag2196 Product name: Recombinant human RGS5 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-181 aa of BC030059 Sequence: MCKGLAALPHSCLERAKEIKIKLGILLQKPDSVGDLVIPYNEKPEKPAKTQKTSLDEALQWRDSLDKLLQNNYGLASFKSFLKSEFSEENLEFWIACEDYKKIKSPAKMAEKAKQIYEEFIQTEAPKEVNIDHFTKDITMKNLVEPSLSSFDMAQKRIHALMEKDSLPRFVRSEFYQELIK Predict reactive species
Full Name	regulator of G-protein signaling 5
Calculated Molecular Weight	181 aa, 21 kDa
Observed Molecular Weight	42 kDa
GenBank Accession Number	BC030059
Gene Symbol	RGS5
Gene ID (NCBI)	8490
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	O15539
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RGS5 inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. It binds to G(i)-alpha and G(o)-alpha, but not to G(s)-alpha. The 42kDa band recognized by this antibody is a RGS5-RGS5 dimer. (PMID:17762159)