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ATXN10 Recombinant monoclonal antibody (88414-1-RR)

ATXN10 Recombinant monoclonal antibody

ATXN10 Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 88414-1-RR

Clone No.260934C7

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

ataxin 10, Ataxin-10, E46L, SCA10

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

SKU:

-/ -

Freight/Packing: -

Quantity

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WB analysis using 88414-1-RR
Various lysates were subjected to SDS PAGE followed by western blot with 88414-1-RR (ATXN10 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis using 88414-1-RR
Various lysates were subjected to SDS PAGE followed by western blot with 88414-1-RR (ATXN10 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Overview

Tested Applications
Product Information
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in

HeLa cells, mouse stomach tissue, HepG2 cells, HEK-293 cells, A431 cells, BxPC-3 cells, rat stomach tissue

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

88414-1-RR targets ATXN10 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Ag8259 Product name: Recombinant human ATXN10 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 120-475 aa of BC007508 Sequence: ELRVEQESLLTAFRCGLQFLGNIASRNEDSQSIVWVHAFPELFLSCLNHPDKKIVAYSSMILFTSLNHERMKELEENLNIAIDVIDAYQKHPESEWPFLIITDLFLKSPELVQAMFPKLNNQERVTLLDLMIAKITSDEPLTKDDIPVFLRHAELIASTFVDQCKTVLKLASEEPPDDEEALATIRLLDVLCEMTVNTELLGYLQVFPGLLERVIDLLRVIHVAGKETTNIFSNCGCVRAEGDISNVANGFKSHLIRLIGNLCYKNKDNQDKVNELDGIPLILDNCNISDSNPFLTQWVIYAIRNLTEDNSQNQDLIAKMEEQGLADASLLKKVGFEVEKKGEKLILKSTRDTPKP Predict reactive species
Full Name	ataxin 10
Calculated Molecular Weight	53 kDa
Observed Molecular Weight	45-50 kDa
GenBank Accession Number	BC007508
Gene Symbol	ATXN10
Gene ID (NCBI)	25814
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q9UBB4
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ATXN10, also named as SCA10, is a 475 amino acid protein, which belongs to the ataxin-10 family. ATXN10 is expressed in the central nervous system.ATXN10 is necessary for the survival of cerebellar neurons. ATXN10 may play a role in the maintenance of a critical intracellular glycosylation level and homeostasis.