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Calbindin-D28k Recombinant monoclonal antibody (88615-1-RR)

Calbindin-D28k Recombinant monoclonal antibody

Calbindin-D28k Uni-rAb^® Recombinant Antibody for WB, IHC, IF-P, ELISA

Cat No. 88615-1-RR

Clone No. 261340A1

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF-P, ELISA

Synonyms

CALB1, Calbindin, CALB, calbindin 1, 28kDa, Calbindin D28

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

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Filter: AllWBIHCIF-PAffinity

WB analysis using 88615-1-RR
Various lysates were subjected to SDS PAGE followed by western blot with 88615-1-RR (Calbindin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of mouse cerebellum using 88615-1-RR
mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 88615-1-RR (Calbindin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of pig brain using 88615-1-RR
pig brain tissue were subjected to SDS PAGE followed by western blot with 88615-1-RR (Calbindin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of rat cerebellum using 88615-1-RR
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 88615-1-RR (Calbindin-D28k antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of rat cerebellum using 88615-1-RR
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 88615-1-RR (Calbindin-D28k antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse cerebellum using 88615-1-RR
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 88615-1-RR (Calbindin-D28k antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse cerebellum using 88615-1-RR
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 88615-1-RR (Calbindin-D28k antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of mouse cerebellum using 88615-1-RR
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse cerebellum tissue using Calbindin-D28k antibody (88615-1-RR, Clone: 261340A1 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
Affinity of 88615-1-RR
Biolayer interferometry (BLI) kinetic assays of 88615-1-RR against Human Calbindin-D28k were performed. The affinity constant is 2.13 nM.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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What's the best dilution for Calbindin-D28k in ?

Overview

Tested Applications
Product Information
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	mouse brain tissue, mouse cerebellum tissue, pig brain tissue, rat brain tissue, fetal human brain tissue
Positive IHC detected in	rat cerebellum tissue, mouse cerebellum tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	mouse cerebellum tissue

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunohistochemistry (IHC)	IHC : 1:500-1:2000
Immunofluorescence (IF)-P	IF-P : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

88615-1-RR targets Calbindin-D28k in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity	human, mouse, rat, pig
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Ag5861 Product name: Recombinant human Calbindin protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 110-261 aa of BC006478 Sequence: YDTDHSGFIETEELKNFLKDLLEKANKTVDDTKLAEYTDLMLKLFDSNNDGKLELTEMARLLPVQENFLLKFQGIKMCGKEFNKAFELYDQDGNGYIDENELDALLKDLCEKNKQDLDINNITTYKKNIMALSDGGKLYRTDLALILCAGDN Predict reactive species
Full Name	calbindin 1, 28kDa
Calculated Molecular Weight	30 kDa
Observed Molecular Weight	28 kDa
GenBank Accession Number	BC006478
Gene Symbol	Calbindin
Gene ID (NCBI)	793
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P05937
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Calbindin-D28k is a member of the calcium-binding protein superfamily that includes calmodulin and troponin C. It is a cytosolic calcium binding protein highly expressed in the distal tubule, intestines, central nervous system, primary murine osteoblast cells and in several other organs. It plays an important role in the intracellular calcium homeostasis, its strong buffering capacity prevents cytotoxic effect of high concentration of free calcium in kidney, brain, pancreas, and intestine.

Protocols

Product Specific Protocols
IF protocol for Calbindin-D28k antibody 88615-1-RR	Download protocol
IHC protocol for Calbindin-D28k antibody 88615-1-RR	Download protocol
WB protocol for Calbindin-D28k antibody 88615-1-RR	Download protocol