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Gamma Catenin Recombinant monoclonal antibody (88895-2-RR)

Gamma Catenin Recombinant monoclonal antibody

Gamma Catenin Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 88895-2-RR

Clone No.261780E6

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, canine

Applications

WB, ELISA

Synonyms

JUP, JUP/gamma Catenin, γ-Catenin, ARVD12, Catenin gamma

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

SKU:

-/ -

Freight/Packing: -

Quantity

- +

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Filter: AllWBIF/ICC

WB analysis using 88895-2-RR
Various lysates were subjected to SDS PAGE followed by western blot with 88895-2-RR (JUP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis using 88895-2-RR
Various lysates were subjected to SDS PAGE followed by western blot with 88895-2-RR (JUP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis using 88895-2-RR
C2C12 cells lysates were subjected to SDS PAGE followed by western blot with 88895-2-RR (JUP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of HT-29 using 88895-2-RR
Immunofluorescent analysis of (4% PFA) fixed HT-29 cells using Gamma Catenin antibody (88895-2-RR, Clone: 261780E6 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Overview

Tested Applications
Product Information
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	HEK-293 cells, MDCK cells, C2C12 cells, A431 cells, MCF-7 cells, MDA-MB-231 cells, mouse heart tissue
Positive IF/ICC detected in	HT-29 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunofluorescence (IF)/ICC	IF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

88895-2-RR targets Gamma Catenin in WB, ELISA applications and shows reactivity with human, mouse, canine samples.

Tested Reactivity	human, mouse, canine
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Ag1627 Product name: Recombinant human JUP protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 396-745 aa of BC011865 Sequence: LVNQLSVDDVNVLTCATGTLSNLTCNNSKNKTLVTQNSGVEALIHAILRAGDKDDITEPAVCALRHLTSRHPEAEMAQNSVRLNYGIPAIVKLLNQPNQWPLVKATIGLIRNLALCPANHAPLQEAAVIPRLVQLLVKAHQDAQRHVAAGTQQPYTDGVRMEEIVEGCTGALHILARDPMNRMEIFRLNTIPLFVQLLYSSVENIQRVAAGVLCELAQDKEAADAIDAEGASAPLMELLHSRNEGTATYAAAVLFRISEDKNPDYRKRVSVELTNSLFKHDPAAWEAAQSMIPINEPYGDDLDATYRPMYSSDVPLDPLEMHMDMDGDYPIDTYSDGLRPPYPTADHMLA Predict reactive species
Full Name	junction plakoglobin
Calculated Molecular Weight	82 kDa
Observed Molecular Weight	82 kDa
GenBank Accession Number	BC011865
Gene Symbol	Gamma Catenin
Gene ID (NCBI)	3728
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P14923
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Plakoglobin (γ-catenin), a member of the Armadillo proteins, plays important roles in cell adhesion with α-catenin and β-catenin, is involved in Wnt signaling, and is translocated into the nucleus and binds LEF1. Plakoglobin is a major cytoplasmic component of both desmosomes and adherens junctions, and is the only known constituent common to submembranous plaques in both of these structures, which are located at the intercalated disc (ICD) of cardiomyocytes. Plakoglobin links cadherins to the actin cytoskeleton. Mutations in plakoglobin are associated with arrhythmogenic right ventricular dysplasia and Pemphigus vulgaris.