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TCF4/TCF7L2 Recombinant monoclonal antibody

TCF4/TCF7L2 Uni-rAb® Recombinant Antibody for WB, IHC, IP, ELISA

Cat No. 89174-1-RR
Clone No. 262241D1
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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IP, ELISA

Synonyms

TCF7L2, HMG box transcription factor 4, hTCF 4, hTCF-4, T cell factor 4

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
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  • Western Blot (WB) analysis of various lysates using TCF4/TCF7L2 Recombinant monoclonal antibody (89174-1-RR)

    WB analysis using 89174-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 89174-1-RR (TCF7L2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

    This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
  • Immunoprecipitation (IP) experiment of HEK-293 cells using TCF4/TCF7L2 Recombinant monoclonal antibody (89174-1-RR)

    IP experiment of HEK-293 using 89174-1-RR

    IP result of anti-TCF4/TCF7L2 (IP:89174-1-RR, 4ug; Detection:89174-1-RR 1:2000) with HEK-293 cells lysate 1800 ug.

    This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
  • Immunohistochemistry (IHC) staining of human colon tissue using TCF4/TCF7L2 Recombinant monoclonal antibody (89174-1-RR)

    IHC staining of human colon using 89174-1-RR

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 89174-1-RR (TCF4/TCF7L2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
  • Immunohistochemistry (IHC) staining of human colon tissue using TCF4/TCF7L2 Recombinant monoclonal antibody (89174-1-RR)

    IHC staining of human colon using 89174-1-RR

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 89174-1-RR (TCF4/TCF7L2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
  • Affinity of 89174-1-RR

    Affinity of 89174-1-RR

    Biolayer interferometry (BLI) kinetic assays of 89174-1-RR against Human TCF4/TCF7L2 were performed. The affinity constant is 1.01 nM.

    This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
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Tested Applications

Positive WB detected inHEK-293 cells, L02 cells, HepG2 cells, HCT 116 cells, SW480 cells, mouse small intestine tissue, mouse colon tissue
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

89174-1-RR targets TCF4/TCF7L2 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag4729

Product name: Recombinant human TCF7L2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-355 aa of BC032656

Sequence: MPQLNGGGGDDLGANDELISFKDEGEQEEKSSENSSAERDLADVKSSLVNESETNQNSSSDSEAERRPPPRSESFRDKSRESLEEAAKRQDGGLFKGPPYPGYPFIMIPDLTSPYLPNGSLSPTARTYLQMKWPLLDVQAGSLQSRQALKDARSPSPAHIVSNKVPVVQHPHHVHPLTPLITYSNEHFTPGNPPPHLPADVDPKTGIPRPPHPPDISPYYPLSPGTVGQIPHPLGWQGQPVYPITTGGFRHPYPTALTVNASMSRFPPHMVPPHHTLHTTGIPHPAIVTPTVKQESSQSDVGSLHSSKHQDSKKEEEKKKPHIKKPLNAFMLYMKEMRAKVVAECTLKESAAINQ

Predict reactive species
Full Name transcription factor 7-like 2 (T-cell specific, HMG-box)
Calculated Molecular Weight 619 aa, 68 kDa
Observed Molecular Weight60, 75 kDa
GenBank Accession NumberBC032656
Gene Symbol TCF4
Gene ID (NCBI) 6934
Conjugate Unconjugated
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ9NQB0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TCF7L2 encodes an HMG-box transcription factor and key effector of canonical Wnt/β-catenin signaling, regulating development and tissue homeostasis. It is the strongest genetic susceptibility locus for type 2 diabetes. Alternative splicing produces functionally distinct isoforms, and TCF7L2 variants have also been linked to Crohn's disease and cancer, indicating pleiotropic roles in disease.

Protocols

Product Specific Protocols
IHC protocol for TCF4/TCF7L2 antibody 89174-1-RRDownload protocol
IP protocol for TCF4/TCF7L2 antibody 89174-1-RRDownload protocol
WB protocol for TCF4/TCF7L2 antibody 89174-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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