Tested Applications
| Positive FC detected in | anti-CD3/CD28 treated mouse splenocytes |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) | FC : 0.25 ug per 10^6 cells in 100 μl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
98166-1-RR targets PD-L1/CD274 in FC applications and shows reactivity with mouse samples.
| Tested Reactivity | mouse |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Eg0986 Product name: Recombinant Mouse PD-L1/B7-H1 protein (His Tag) Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS Tag: C-6*HIS Domain: 19-239 aa of NM_021893.3 Sequence: FTITAPKDLYVVEYGSNVTMECRFPVERELDLLALVVYWEKEDEQVIQFVAGEEDLKPQHSNFRGRASLPKDQLLKGNAALQITDVKLQDAGVYCCIISYGGADYKRITLKVNAPYRKINQRISVDPATSEHELICQAEGYPEAEVIWTNSDHQPVSGKRSVTTSRTEGMLLNVTSSLRVNATANDVFYCTFWRSQPGQNHTAELIIPELPATHPPQNRTH Predict reactive species |
| Full Name | CD274 antigen |
| Calculated Molecular Weight | 33 kDa |
| GenBank Accession Number | NM_021893.3 |
| Gene Symbol | PD-L1 |
| Gene ID (NCBI) | 60533 |
| RRID | AB_3672309 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9EP73 |
| Storage Buffer | PBS with 0.09% sodium azide, pH 7.3. |
| Storage Conditions | Store at 2 - 8°C. Stable for one year after shipment. |
Background Information
Programmed cell death ligand 1 (PD-L1, CD274, or B7-H1) is the first member of B7 family to be discovered. B7 family molecules are type I transmembrane proteins belonging to the immunoglobulin superfamily. In concert with their CD28 family receptors, the B7s are key regulators of the adaptive immune response. PD-L1 is suggested as a negative regulator of T and B cells, and plays an important role in mediating tolerance of lymphocytes to self-antigens. It is also involved in the costimulatory signal, essential for T-cell proliferation and production of IL-10 and IFN gamma, in an IL-2-dependent and a PD-1-independent manner.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for PD-L1/CD274 antibody 98166-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Anti-Mouse PD-L1/CD274 Rabbit Recombinant Antibody (240993E1), catalog number 98166-1-RR, has 3 total citations. These appear in Acta Pharmaceutica Sinica B, Phytomedicine, and World Journal of Oncology. The associated research fields include antitumor photoimmunotherapy involving pyroptosis and immunogenic cell death, discovery of natural product-derived PD-L1 inhibitors for cancer immunotherapy, and adjuvanted vaccine strategies synergizing with anti-PD-1 therapy to enhance antitumor immunity in murine lung cancer models.
| Species | Application | Title |
|---|---|---|
Acta Pharm Sin B Extracellular vesicles-derived hybrid biomimetic nanoplatforms camouflaged Golgi apparatus-targeted aggregation induced emission photosensitizer to elicit pyroptosis and immunogenic cell death for efficient antitumor photoimmunotherapy. | ||
Phytomedicine Discovery of natural product-derived rosavin as a programmed death-ligand 1 inhibitor for cancer immunotherapy. | ||
World J Oncol RAD001-Adjuvanted Influenza Vaccine Synergizes With Anti-Programmed Cell Death Protein 1 Therapy to Enhance Antitumor Immunity in Programmed Cell Death Ligand 1-Low Murine Lung Cancer. |
