FC experiment of mouse bone marrow cells using 98225-1-RR
1x10^6 mouse bone marrow cells were surface stained with 0.25 ug Anti-Mouse SIRP alpha/CD172a Rabbit Recombinant Antibody (98225-1-RR, Clone: 241794D9) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Mouse CD11b. Cells were not fixed.
1x10^6 mouse bone marrow cells were surface stained with 0.25 ug Anti-Mouse SIRP alpha/CD172a Rabbit Recombinant Antibody (98225-1-RR, Clone: 241794D9) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Mouse CD11b. Cells were not fixed.
FC experiment of mouse bone marrow cells using 98225-1-RR
1x10^6 mouse bone marrow cells were surface stained with 0.25 ug Anti-Mouse SIRP alpha/CD172a Rabbit Recombinant Antibody (98225-1-RR, Clone: 241794D9) (red) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.
1x10^6 mouse bone marrow cells were surface stained with 0.25 ug Anti-Mouse SIRP alpha/CD172a Rabbit Recombinant Antibody (98225-1-RR, Clone: 241794D9) (red) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.
Affinity and Kinetic Characterization of 98225-1-RR
Biolayer interferometry (BLl) kinetic assays of 98225-1-RR against Mouse SIRP Alpha/CD172a were performed. The affinity constant is below 1 pM.
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This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
Product Information
98225-1-RR targets SIRP Alpha/CD172a in FC applications and shows reactivity with mouse samples.
Store at 2 - 8°C. Stable for one year after shipment.
Background Information
SIRP Alpha, also known as CD172a, SHPS-1, and BIT, belongs to the SIRP family. SIRP Alpha is a transmembrane glycoprotein expressed explicitly on myeloid cells and provides a "do not eat me" signal after engaging with integrin-associated protein CD47 on tumor cells (PMID: 36419386). SIRP Alpha shows heterogeneity in molecular weight (~65-120 kDa) in various tissues due to differential glycosylation (PMID: 18051954).
Protocols
Product Specific Protocols
FC protocol for SIRP Alpha/CD172a antibody 98225-1-RR
FC experiment of mouse bone marrow cells using 98225-1-RR
1x10^6 mouse bone marrow cells were surface stained with 0.25 ug Anti-Mouse SIRP alpha/CD172a Rabbit Recombinant Antibody (98225-1-RR, Clone: 241794D9) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Mouse CD11b. Cells were not fixed.
FC experiment of mouse bone marrow cells using 98225-1-RR
1x10^6 mouse bone marrow cells were surface stained with 0.25 ug Anti-Mouse SIRP alpha/CD172a Rabbit Recombinant Antibody (98225-1-RR, Clone: 241794D9) (red) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.
AFFINITY Figures
Affinity and Kinetic Characterization of 98225-1-RR
Biolayer interferometry (BLl) kinetic assays of 98225-1-RR against Mouse SIRP Alpha/CD172a were performed. The affinity constant is below 1 pM.
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
SIRP Alpha/CD172a Recombinant antibody
Catalog Number
98225-1-RR
Citations
-
Dilutions
Applications
FC
Reactivity
mouse
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
