CoraLite® Plus 405 Anti-Mouse SIRP Alpha/CD172a Rabbit Recombinant Antibody

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  FC experiment of mouse bone marrow cells using CL405-98225

  1x10^6 mouse bone marrow cells were surface stained with CoraLite® Plus 647 Anti-Mouse CD11b (M1/70) (CL647-65055, Clone: M1/70), and 0.25 ug CoraLite® Plus 405 Anti-Mouse SIRP Alpha/CD172a Rabbit RecAb (CL405-98225, Clone:241794D9) or 0.25 ug CoraLite® Plus 405 Rabbit IgG Isotype Control RecAb (CL405-98136, Clone: 240953C9). Cells were not fixed.

• 

  FC experiment of mouse bone marrow cells using CL405-98225

  1x10^6 mouse bone marrow cells were surface stained with 0.25 ug CoraLite® Plus 405 Anti-Mouse SIRP Alpha/CD172a Rabbit RecAb (CL405-98225, Clone: 241794D9) (red) or 0.25 ug CoraLite® Plus 405 Rabbit IgG Isotype Control RecAb (CL405-98136, Clone: 240953C9) (blue). Cells were not fixed.

SIRP Alpha/CD172a Uni-rAb^TM Recombinant Antibody for FC

Cat No. CL405-98225

Clone No.241794D9

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC

Synonyms

Inhibitory receptor SHPS-1, CD172a, CD172 antigen-like family member A, Brain Ig-like molecule with tyrosine-based activation motifs, Bit

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Tested Applications

• Positive FC detected in: mouse bone marrow cells

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in 100 μl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL405-98225 targets SIRP Alpha/CD172a in FC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: signal-regulatory protein alpha
• Calculated Molecular Weight: 56 kDa
• GenBank Accession Number: NM_007547.4
• Gene Symbol: Sirpa
• Gene ID (NCBI): 19261
• Conjugate: CoraLite® Plus 405 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 399 nm / 422 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P97797-2
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

SIRP Alpha, also known as CD172a, SHPS-1, and BIT, belongs to the SIRP family. SIRP Alpha is a transmembrane glycoprotein expressed explicitly on myeloid cells and provides a "do not eat me" signal after engaging with integrin-associated protein CD47 on tumor cells (PMID: 36419386). SIRP Alpha shows heterogeneity in molecular weight (~65-120 kDa) in various tissues due to differential glycosylation (PMID: 18051954).