Tested Applications
Positive FC detected in | mouse bone marrow cells |
Recommended dilution
Application | Dilution |
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This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
CL405-98225 targets SIRP Alpha/CD172a in FC applications and shows reactivity with mouse samples.
Tested Reactivity | mouse |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | Recombinant protein Predict reactive species |
Full Name | signal-regulatory protein alpha |
Calculated Molecular Weight | 56 kDa |
GenBank Accession Number | NM_007547.4 |
Gene Symbol | Sirpa |
Gene ID (NCBI) | 19261 |
Conjugate | CoraLite® Plus 405 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 399 nm / 422 nm |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P97797-2 |
Storage Buffer | PBS with 0.09% sodium azide, pH 7.3. |
Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Background Information
SIRP Alpha, also known as CD172a, SHPS-1, and BIT, belongs to the SIRP family. SIRP Alpha is a transmembrane glycoprotein expressed explicitly on myeloid cells and provides a "do not eat me" signal after engaging with integrin-associated protein CD47 on tumor cells (PMID: 36419386). SIRP Alpha shows heterogeneity in molecular weight (~65-120 kDa) in various tissues due to differential glycosylation (PMID: 18051954).
Protocols
Product Specific Protocols | |
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FC protocol for CL Plus 405 SIRP Alpha/CD172a antibody CL405-98225 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |