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Anti-Human IL-2RG/CD132 Rabbit Recombinant Antibody (98234-1-RR)

Anti-Human IL-2RG/CD132 Rabbit Recombinant Antibody

IL-2RG/CD132 Uni-rAb^® Recombinant Antibody for FC

Cat No. 98234-1-RR

Clone No. 242020H6

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Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Synonyms

IL2RG, 242020H6, CD132, gammaC, IL 2 receptor subunit gamma

Formulation: PBS, Azide

PBS, Azide

PBS Only

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

SKU:

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Quantity

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FC experiment of human PBMCs using 98234-1-RR
1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human IL-2RG/CD132 Rabbit RecAb (98234-1-RR, Clone: 242020H6) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with APC Anti-Human CD4 Rabbit Recombinant Antibody (APC-98042, Clone: 240427E12). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
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1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human IL-2RG/CD132 Rabbit RecAb (98234-1-RR, Clone: 242020H6) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with APC Anti-Human CD4 Rabbit Recombinant Antibody (APC-98042, Clone: 240427E12). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.
FC experiment of human PBMCs using 98234-1-RR
1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human IL-2RG/CD132 Rabbit RecAb (98234-1-RR, Clone: 242020H6) (red) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
Affinity and Kinetic Characterization of 98234-1-RR
Biolayer interferometry (BLl) kinetic assays of 98234-1-RR against Human IL-2RG/CD132 were performed. The affinity constant is 1.55 nM.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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What's the best dilution for IL-2RG/CD132 in ?

Overview

Tested Applications
Product Information
Protocols

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MSDS SDS

Tested Applications

Positive FC detected in

human PBMCs

Recommended dilution

Application	Dilution
Flow Cytometry (FC)	FC : 0.25 ug per 10^6 cells in a 100 µl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

98234-1-RR targets IL-2RG/CD132 in FC applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Eg1705 Product name: Recombinant Human IL-2 R gamma/CD132 protein (His Tag) Source: mammalian cells-derived, pHZ-KIsec-C-6HIS Tag: C-6HIS Domain: 23-254 aa of NM_000206.3 Sequence: LNTTILTPNGNEDTTADFFLTTMPTDSLSVSTLPLPEVQCFVFNVEYMNCTWNSSSEPQPTNLTLHYWYKNSDNDKVQKCSHYLFSEEITSGCQLQKKEIHLYQTFVVQLQDPREPRRQATQMLKLQNLVIPWAPENLTLHKLSESQLELNWNNRFLNHCLEHLVQYRTDWDHSWTEQSVDYRHKFSLPSVDGQKRYTFRVRSRFNPLCGSAQHWSEWSHPIHWGSNTSKEN Predict reactive species
Full Name	interleukin 2 receptor, gamma (severe combined immunodeficiency)
Calculated Molecular Weight	NM_000206.3
GenBank Accession Number	NM_000206.3
Gene Symbol	IL2RG
Gene ID (NCBI)	3561
RRID	AB_3745901
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P31785-1
Storage Buffer	PBS with 0.09% sodium azide, pH 7.3.
Storage Conditions	Store at 2 - 8°C. Stable for one year after shipment.

Background Information

The interleukin-2 receptor (IL-2R) is a heterotrimeric receptor consisting of three subunits, alpha (IL-2RA), beta (IL-2RB), and gamma (IL-2RG). IL-2RG, also known as CD132, is an important signaling component of many interleukin receptors, including those of interleukin -2, -4, -7 and -21, and is thus referred to as the common gamma chain. Mutations in the gene of IL-2RG cause X-linked severe combined immunodeficiency (XSCID), as well as X-linked combined immunodeficiency (XCID), a less severe immunodeficiency disorder.