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Anti-Human HVEM/TNFRSF14 Rabbit Recombinant Antibody (98362-2-RR)

Anti-Human HVEM/TNFRSF14 Rabbit Recombinant Antibody

HVEM/TNFRSF14 Uni-rAb^® Recombinant Antibody for FC

Cat No. 98362-2-RR

Clone No.250728C11

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Synonyms

TNFRSF14, ATAR, CD270, Herpes virus entry mediator A, Herpesvirus entry mediator A

Formulation: PBS, Azide

PBS, Azide

PBS Only

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

SKU:

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Freight/Packing: -

Quantity

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Filter: AllFCAffinity

FC experiment of human peripheral blood leukocytes using 98362-2-RR
1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human HVEM/TNFRSF14 Rabbit RecAb (98362-2-RR, Clone: 250728C11) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
FC experiment of human peripheral blood leukocytes using 98362-2-RR
1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human HVEM/TNFRSF14 Rabbit RecAb (98362-2-RR, Clone: 250728C11) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
Affinity and Kinetic Characterization of 98362-2-RR
Biolayer interferometry (BLl) kinetic assays of 98362-2-RR against Human HVEM/TNFRSF14 were performed. The affinity constant is 2.22 nM.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Overview

Tested Applications
Product Information
Protocols

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MSDS SDS

Tested Applications

Positive FC detected in

human peripheral blood leukocytes

Recommended dilution

Application	Dilution
Flow Cytometry (FC)	FC : 0.25 ug per 10^6 cells in a 100 µl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

98362-2-RR targets HVEM/TNFRSF14 in FC applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Eg1275 Product name: Recombinant Human HVEM/TNFRSF14 protein (His Tag) Source: mammalian cells-derived, pHZ-KIsec-C-6HIS Tag: C-6HIS Domain: 39-202 aa of BC002794 Sequence: LPSCKEDEYPVGSECCPKCSPGYRVKEACGELTGTVCEPCPPGTYIAHLNGLSKCLQCQMCDPAMGLRASRNCSRTENAVCGCSPGHFCIVQDGDHCAACRAYATSSPGQRVQKGGTESQDTLCQNCPPGTFSPNGTLEECQHQTKCSWLVTKAGAGTSSSHWV Predict reactive species
Full Name	tumor necrosis factor receptor superfamily, member 14 (herpesvirus entry mediator)
Calculated Molecular Weight	30 kDa
GenBank Accession Number	BC002794
Gene Symbol	TNFRSF14
Gene ID (NCBI)	8764
RRID	AB_3746012
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q92956
Storage Buffer	PBS with 0.09% sodium azide, pH 7.3.
Storage Conditions	Store at 2 - 8°C. Stable for one year after shipment.

Background Information

The mammalian tumor necrosis factor receptor (TNFR) family consists of 10 cell-surface proteins that regulate development and homeostasis of the immune system. Member 14 of TNFR (TNFRSF14, also named as TR2, ATAR, HVEA, HVEM, LIGHTR) was also identified as a mediator of herpesvirus entry into mammalian cells. The cytoplasmic region of TNFRSF14 bound to several members of the TNFR-associated factor (TRAF) family, namely TRAF1, TRAF2, TRAF3, and TRAF5. Transient transfection into human 293 cells caused marked activation of nuclear factor- B (NF- B), a transcriptional regulator of multiple immunomodulatory and inflammatory genes.