Home
Products
Primary Antibodies
Flow Cytometry Antibodies
Anti-Mouse CD134/OX40 Rabbit Recombinant Antibody (98409-2-RR)

Anti-Mouse CD134/OX40 Rabbit Recombinant Antibody

CD134/OX40 Uni-rAb^® Recombinant Antibody for FC

Cat No. 98409-2-RR

Clone No. 242901A7

Review Product

Download datasheet

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

Synonyms

CD134, Tnfrsf4, Ox40, OX40 antigen, OX40L receptor

Formulation: PBS, Azide

PBS, Azide

PBS Only

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

SKU:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

Proteintech Guarantee

Filter: AllFC

FC experiment of BALB/c mouse splenocytes using 98409-2-RR
1x10^6 Untreated or CD3/CD28 treated BALB/c mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD134/OX40 Rabbit RecAb (98409-2-RR, Clone:242901A7) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with APC Anti-Mouse CD4 (GK1.5) (APC-65104, Clone: GK1.5). Cells were not fixed.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
FC experiment of BALB/c mouse splenocytes using 98409-2-RR
1x10^6 CD3/CD28 treated BALB/c mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD134/OX40 Rabbit RecAb (98409-2-RR, Clone:242901A7) (red) and PE-Conjugated Goat Anti-Rabbit IgG(H+L), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). 1x10^6 untreated BALB/c mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD134/OX40 Rabbit RecAb (98409-2-RR, Clone:242901A7) (black, dashed) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
×
1x10^6 CD3/CD28 treated BALB/c mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD134/OX40 Rabbit RecAb (98409-2-RR, Clone:242901A7) (red) and PE-Conjugated Goat Anti-Rabbit IgG(H+L), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). 1x10^6 untreated BALB/c mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD134/OX40 Rabbit RecAb (98409-2-RR, Clone:242901A7) (black, dashed) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

Able AI

What's the best dilution for CD134/OX40 in ?

Overview

Tested Applications
Product Information
Protocols

Print datasheet

MSDS SDS

Tested Applications

Positive FC detected in

CD3/CD28 treated BALB/c mouse splenocytes

Recommended dilution

Application	Dilution
Flow Cytometry (FC)	FC : 0.25 ug per 10^6 cells in 100 μl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

98409-2-RR targets CD134/OX40 in FC applications and shows reactivity with mouse samples.

Tested Reactivity	mouse
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Eg2936 Product name: Recombinant Mouse CD134/OX40 protein (rFc Tag) Source: mammalian cells-derived, V37 Tag: C-rFc Domain: 20-211 aa of NM_011659.2 Sequence: VTARRLNCVKHTYPSGHKCCRECQPGHGMVSRCDHTRDTLCHPCETGFYNEAVNYDTCKQCTQCNHRSGSELKQNCTPTQDTVCRCRPGTQPRQDSGYKLGVDCVPCPPGHFSPGNNQACKPWTNCTLSGKQTRHPASDSLDAVCEDRSLLATLLWETQRPTFRPTTVQSTTVWPRTSELPSPPTLVTPEGP Predict reactive species
Full Name	tumor necrosis factor receptor superfamily, member 4
Calculated Molecular Weight	30 kDa
GenBank Accession Number	NM_011659.2
Gene Symbol	CD134
Gene ID (NCBI)	22163
RRID	AB_3746050
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P47741
Storage Buffer	PBS with 0.09% sodium azide, pH 7.3.
Storage Conditions	Store at 2 - 8°C. Stable for one year after shipment.

Background Information

CD134, also known as OX40 and TNFRSF4, is a member of the TNFR-superfamily of receptors (PMID: 2828930; 9766631). It is a type I transmembrane protein predominantly expressed on activated T cells which include CD4 and CD8 T cells, Th2, Th1, and Th17 cells, as well as regulatory T cells (Tregs) (PMID: 20307208). CD134 is activated by its cognate ligand CD134L (OX40L) and functions as a T cell co-stimulatory molecule (PMID: 26215166). CD134-CD134L interactions have been proposed as a potential therapeutic target for treating autoimmune diseases, cancer and infectious disease (PMID: 26215166; 19426222).