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Anti-Human CD58 Rabbit Recombinant Antibody (98452-5-RR)

Anti-Human CD58 Rabbit Recombinant Antibody

CD58 Uni-rAb^® Recombinant Antibody for FC

Cat No. 98452-5-RR

Clone No. 250250H5

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Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Synonyms

Ag3, CD58 molecule, LFA 3, LFA3, Lymphocyte function-associated antigen 3

Formulation: PBS, Azide

PBS, Azide

PBS Only

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

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FC experiment of human peripheral blood leukocyte using 98452-5-RR
1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human CD58 Rabbit RecAb (98452-5-RR, Clone: 250250H5) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
FC experiment of human peripheral blood leukocyte using 98452-5-RR
1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human CD58 Rabbit RecAb (98452-5-RR, Clone: 250250H5) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Overview

Tested Applications
Product Information
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MSDS SDS

Tested Applications

Positive FC detected in

human peripheral blood leukocytes

Recommended dilution

Application	Dilution
Flow Cytometry (FC)	FC : 0.25 ug per 10^6 cells in a 100 µl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

98452-5-RR targets CD58 in FC applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Eg4236 Product name: Recombinant Human CD58 protein (rFc Tag) (HPLC verified) Source: mammalian cells-derived, pHZ-KIsec-C-rFc Tag: C-rFc Domain: 29-215 aa of BC005930 Sequence: FSQQIYGVVYGNVTFHVPSNVPLKEVLWKKQKDKVAELENSEFRAFSSFKNRVYLDTVSGSLTIYNLTSSDEDEYEMESPNITDTMKFFLYVLESLPSPTLTCALTNGSIEVQCMIPEHYNSHRGLIMYSWDCPMEQCKRNSTSIYFKMENDLPQKIQCTLSNPLFNTTSSIILTTCIPSSGHSRHR Predict reactive species
Full Name	CD58 molecule
Calculated Molecular Weight	28 kDa
GenBank Accession Number	BC005930
Gene Symbol	CD58
Gene ID (NCBI)	965
RRID	AB_3746083
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P19256
Storage Buffer	PBS with 0.09% sodium azide, pH 7.3.
Storage Conditions	Store at 2 - 8°C. Stable for one year after shipment.

Background Information

CD58, also known as lymphocyte function-associated antigen 3 (LFA-3), is a heavily glycosylated protein of 55-70 kDa that is expressed on a broad range of hematopoietic and non-hematopoietic cells. CD58 is involved in immune recognition of tumor cells via binding of the CD2 receptor expressed on cytotoxic T cells.