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Anti-Human LILRB2/CD85d Rabbit Recombinant Antibody (98488-3-RR)

Anti-Human LILRB2/CD85d Rabbit Recombinant Antibody

LILRB2/CD85d Uni-rAb^® Recombinant Antibody for FC

Cat No. 98488-3-RR

Clone No. 250299A6

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Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Synonyms

LILRB2, CD85 antigen-like family member D, CD85d, ILT 4, ILT4

Formulation: PBS, Azide

PBS, Azide

PBS Only

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

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Filter: AllFC

FC experiment of human PBMCs using 98488-3-RR
1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human LILRB2/CD85d Rabbit RecAb (98488-3-RR, Clone:250299A6) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
FC experiment of human PBMCs using 98488-3-RR
1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human LILRB2/CD85d Rabbit RecAb (98488-3-RR, Clone: 250299A6) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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What's the best dilution for LILRB2/CD85d in ?

Overview

Tested Applications
Product Information
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MSDS SDS

Tested Applications

Positive FC detected in

human PBMCs

Recommended dilution

Application	Dilution
Flow Cytometry (FC)	FC : 0.25 ug per 10^6 cells in a 100 µl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

98488-3-RR targets LILRB2/CD85d in FC applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Eg2839 Product name: Recombinant Human LILRB2/CD85d protein (rFc Tag) Source: mammalian cells-derived, V37 Tag: C-rFc Domain: 22-458 aa of NM_005874.4 Sequence: QTGTIPKPTLWAEPDSVITQGSPVTLSCQGSLEAQEYRLYREKKSASWITRIRPELVKNGQFHIPSITWEHTGRYGCQYYSRARWSELSDPLVLVMTGAYPKPTLSAQPSPVVTSGGRVTLQCESQVAFGGFILCKEGEDEHPQCLNSQPHARGSSRAIFSVGPVSPNRRWSHRCYGYDLNSPYVWSSPSDLLELLVPGVSKKPSLSVQPGPVMAPGESLTLQCVSDVGYDRFVLYKEGERDLRQLPGRQPQAGLSQANFTLGPVSRSYGGQYRCYGAHNLSSECSAPSDPLDILITGQIRGTPFISVQPGPTVASGENVTLLCQSWRQFHTFLLTKAGAADAPLRLRSIHEYPKYQAEFPMSPVTSAHAGTYRCYGSLNSDPYLLSHPSEPLELVVSGPSMGSSPPPTGPISTPAGPEDQPLTPTGSDPQSGLGRH Predict reactive species
Full Name	leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains), member 2
Calculated Molecular Weight	65 kDa
GenBank Accession Number	NM_005874.4
Gene Symbol	LILRB2
Gene ID (NCBI)	10288
RRID	AB_3746118
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q8N423-1
Storage Buffer	PBS with 0.09% sodium azide, pH 7.3.
Storage Conditions	Store at 2 - 8°C. Stable for one year after shipment.

Background Information

LILRB2 (Leukocyte immunoglobulin-like receptor subfamily B member 2), also known as ILT4, CD85d or MIR10, is a type I transmembrane glycoprotein that contains four extracellular immunoglobulin domains, a transmembrane domain, and three cytoplasmic immunoreceptor tyrosine-based inhibitory motifs (ITIMs) (PMID: 33928233). LILRB2 is mainly expressed in immunocytes including monocytes, macrophages, and dendritic cells, and transduces negative signals by its association with SHP-1 phosphatase via the ITIMs (PMID: 35717259). LILRB2 can interact with various ligands such as HLA-A, HLA-B, HLA-C, HLA-G, HLA-F, CD1c, CD1d, angiopoietin-like proteins (ANGPTLs), and SEMA4A.